Protein Variants | Comment | Organism |
---|---|---|
additional information | replacement of Tyr7 with eight other amino acids has no effect on the interconversion of bicarbonate and CO2, but in some cases causes enhancements in the rate constant of proton transfer by nearly 10fold. Mutant variants at position 7 show a melting temperature approximately 8°C lower than that of the wild-type enzyme | Homo sapiens |
Y7A | site-directed mutagenesis | Homo sapiens |
Y7D | site-directed mutagenesis | Homo sapiens |
Y7F | site-directed mutagenesis | Homo sapiens |
Y7I | site-directed mutagenesis, replacement of Tyr7 with Ile has no effect on the interconversion of bicarbonate and CO2, but enhances intramolecular proton transfer approximately twofold. No changes occur in the ordered solvent structure in the active-site cavity or in the conformation of the side chain of the proton shuttle His64, while the first 11 residues of the amino-terminal chain in Y7I HCA II assume an alternate conformation compared to the wild-type enzyme | Homo sapiens |
Y7N | site-directed mutagenesis | Homo sapiens |
Y7R | site-directed mutagenesis | Homo sapiens |
Y7S | site-directed mutagenesis | Homo sapiens |
Y7W | site-directed mutagenesis | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required. The side chain of Tyr7 in HCA II extends into the active-site cavity about 7 A from the catalytic zinc atom | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Homo sapiens | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
H2CO3 = CO2 + H2O | the rate limiting step in catalysis of bicarbonate dehydration by HCA II is an intramolecular proton transfer from His64 to the zinc-bound hydroxide | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Homo sapiens | CO2 + H2O | - |
r | |
additional information | the enzyme also shows esterase activity in 4-nitrophenylacetate hydrolysis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase II | - |
Homo sapiens |
HCA II | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profiles for the hydration of CO2 catalyzed by wild-type HCA II and mutant Y7I HCA II, overview | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km/kcat values for 4-nitrophenylacetate hydrolysis by wild-type and mutant enzymes, overview | Homo sapiens |