Literature summary for 4.2.1.1 extracted from

Avvaru, B.S.; Arenas, D.J.; Tu, C.; Tanner, D.B.; McKenna, R.; Silverman, D.N.
Comparison of solution and crystal properties of Co(II)-substituted human carbonic anhydrase II (2010), Arch. Biochem. Biophys., 502, 53-59.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of HCA II in Escherichia coli strain BL21	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant HCA II, crystals are prepared at pH 6.0, pH 8.5, and pH 11.0, hanging drop vapor diffusion method, crystal preparation from apo-Co(II)-HCA II by mixing 0.005 ml of protein solution with 0.005 ml of precipitant solution, containing 1.4 M sodium citrate, and 100 mM Tris-HCl, pH 9.0, at room temperature, and soaking of the crystals in 100 mM CoCl2, 1.4 M sodium citrate, 50 mM Tris-Cl, 50 mM 3-(cyclohexylamino)propanesulfonic acid with pH values of 6.0, 8.5 and 11.0, respectively, 2-3days, X-ray diffraction structure determination and analysis at 1.5-1.6 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant HCA II, crystals are prepared at pH 6.0, pH 8.5, and pH 11.0, hanging drop vapor diffusion method, crystal preparation from apo-Co(II)-HCA II by mixing 0.005 ml of protein solution with 0.005 ml of precipitant solution, containing 1.4 M sodium citrate, and 100 mM Tris-HCl, pH 9.0, at room temperature, and soaking of the crystals in 100 mM CoCl2, 1.4 M sodium citrate, 50 mM Tris-Cl, 50 mM 3-(cyclohexylamino)propanesulfonic acid with pH values of 6.0, 8.5 and 11.0, respectively, 2-3days, X-ray diffraction structure determination and analysis at 1.5-1.6 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition of HCA II by citrate and no conformational changes of amino-acid side chains as a result of the use of citrate at pH 6.0-11.0	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	removal of Zn2+ and preparation of Co(II)-substituted carbonic anhydrase II, Co(II)-HCA II. Change in metal coordination from tetrahedral at pH 11.0 to a coordination consistent with a mixed population of both tetrahedral and penta-coordinate at pH 8.5 to an octahedral geometry characteristic of the oxidized enzyme Co(III)-HCA II at pH 6.0	Homo sapiens
Zn2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Homo sapiens	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00918	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HCA II from Escherichia coli strain BL21	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2CO3	-	Homo sapiens	CO2 + H2O	-	r

Synonyms

Synonyms	Comment	Organism
carbonic anhydrase II	-	Homo sapiens
HCA II	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	change in metal coordination from tetrahedral at pH 11.0 to a coordination consistent with a mixed population of both tetrahedral and penta-coordinate at pH 8.5 to an octahedral geometry characteristic of the oxidized enzyme Co(III)-HCA II at pH 6.0	Homo sapiens
6	11	-	Homo sapiens