Literature summary for 4.2.1.1 extracted from

Genis, C.; Sippel, K.H.; Case, N.; Cao, W.; Avvaru, B.S.; Tartaglia, L.J.; Govindasamy, L.; Tu, C.; Agbandje-McKenna, M.; Silverman, D.N.; Rosser, C.J.; McKenna, R.
Design of a carbonic anhydrase IX active-site mimic to screen inhibitors for possible anticancer properties (2009), Biochemistry, 48, 1322-1331.

Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of carbonic anhydrase II and carbonic anhydrase IX mimic are grown at room temperature using the hanging drop vapour diffusion method	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A65S	the mutation can cause variations in binding affinity of small molecule inhibitors	Homo sapiens
N67Q	the mutation can cause variations in binding affinity of small molecule inhibitors	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
acetazolamide	-	Homo sapiens
benzolamide	-	Homo sapiens
chlorzolamide	potent inhibitor	Homo sapiens
ethoxzolamide	-	Homo sapiens
methazolamide	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00918	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Homo sapiens	H2CO3	-	r

Synonyms

Synonyms	Comment	Organism
CA II	-	Homo sapiens
CA IX	-	Homo sapiens
carbonic anhydrase II	-	Homo sapiens
carbonic anhydrase IX	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000001	-	ethoxzolamide	Ki less than 0.000001 mM wild type carbonic anhydrase II	Homo sapiens
0.000001	-	chlorzolamide	wild type carbonic anhydrase IX	Homo sapiens
0.000002	-	chlorzolamide	wild type carbonic anhydrase II	Homo sapiens
0.000003	-	acetazolamide	wild type carbonic anhydrase IX	Homo sapiens
0.000007	-	benzolamide	wild type carbonic anhydrase II	Homo sapiens
0.000009	-	methazolamide	wild type carbonic anhydrase IX	Homo sapiens
0.000009	-	benzolamide	wild type carbonic anhydrase IX	Homo sapiens
0.0000101	-	acetazolamide	wild type carbonic anhydrase II	Homo sapiens
0.0000118	-	methazolamide	wild type carbonic anhydrase II	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)