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Literature summary for 4.2.1.1 extracted from

  • Zheng, J.; Avvaru, B.S.; Tu, C.; McKenna, R.; Silverman, D.N.
    Role of hydrophilic residues in proton transfer during catalysis by human carbonic anhydrase II (2008), Biochemistry, 47, 12028-12036.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants are crystallized by hanging drop vapour diffusion method, with 50 mM Tris-HCl (pH 9.0) and 1.3 M sodium citrate at 20°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
N62A the mutant shows a predominantly inward orientation of the side chain of His64 Homo sapiens
N62D the mutant shows a predominantly outward orientation of the side chain of His64 Homo sapiens
N62L the mutant shows a predominantly inward orientation of the side chain of His64 Homo sapiens
N62T the mutant shows both inward and outward orientation of the side chain of His64 like the wild type enzyme Homo sapiens
N62V the mutant shows a predominantly inward orientation of the side chain of His64 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Homo sapiens
-
 H2CO3
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P00918
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
CO2 + H2O
-
 Homo sapiens H2CO3
-
 r

Synonyms

Synonyms Comment Organism
CA II
-
 Homo sapiens
carbonic anhydrase II
-
 Homo sapiens