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Literature summary for 4.2.1.1 extracted from

  • Smith, K.S.; Ingram-Smith, C.; Ferry, J.G.
    Roles of the conserved aspartate and arginine in the catalytic mechanism of an archaeal beta-class carbonic anhydrase (2002), J. Bacteriol., 184, 4240-4245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and variant carbonic anhydrases heterologously produced in Escherichia coli BL21(DE3) Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
D34A site-directed mutagenesis Methanothermobacter thermautotrophicus
D34A/R36A site-directed mutagenesis Methanothermobacter thermautotrophicus
D34E site-directed mutagenesis Methanothermobacter thermautotrophicus
R36A site-directed mutagenesis Methanothermobacter thermautotrophicus
R36K site-directed mutagenesis Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 CO2 pH 8.5, 25°C, mutant D34E Methanothermobacter thermautotrophicus
2.7
-
 CO2 pH 8.5, 25°C, wild-type, MOPS buffer Methanothermobacter thermautotrophicus
2.9
-
 CO2 pH 8.5, 25°C, wild-type Methanothermobacter thermautotrophicus
3.5
-
 CO2 pH 8.5, 25°C, mutant D34A, MOPS buffer Methanothermobacter thermautotrophicus
3.6
-
 CO2 pH 8.5, 25°C, mutant D34A Methanothermobacter thermautotrophicus
4.2
-
 CO2 pH 8.5, 25°C, wild-type, imidazole buffer Methanothermobacter thermautotrophicus
5.8
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer Methanothermobacter thermautotrophicus
7.2
-
 CO2 pH 8.5, 25°C, mutant R36K Methanothermobacter thermautotrophicus
9.2
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer Methanothermobacter thermautotrophicus
15.3
-
 CO2 pH 8.5, 25°C, mutant D34A, imidazole buffer Methanothermobacter thermautotrophicus
35.3
-
 CO2 pH 8.5, 25°C, mutant R36A, MOPS buffer Methanothermobacter thermautotrophicus
36.2
-
 CO2 pH 8.5, 25°C, mutant R36A Methanothermobacter thermautotrophicus
47.1
-
 CO2 pH 8.5, 25°C, mutant R36A, imidazole buffer Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-hydroxide mechanism of catalysis Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Methanothermobacter thermautotrophicus
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
 Methanothermobacter thermautotrophicus CO2 + H2O
-
 r

Synonyms

Synonyms Comment Organism
beta-class carbonic anhydrase
-
 Methanothermobacter thermautotrophicus
Cab
-
 Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
 CO2 pH 8.5, 25°C, mutant R36A, MOPS buffer Methanothermobacter thermautotrophicus
30
-
 CO2 pH 8.5, 25°C, mutant R36A, imidazole buffer Methanothermobacter thermautotrophicus
46
-
 CO2 pH 8.5, 25°C, mutant R36A Methanothermobacter thermautotrophicus
92
-
 CO2 pH 8.5, 25°C, mutant R36K Methanothermobacter thermautotrophicus
260
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, MOPS buffer Methanothermobacter thermautotrophicus
520
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A Methanothermobacter thermautotrophicus
1100
-
 CO2 pH 8.5, 25°C, mutant D34A, MOPS buffer Methanothermobacter thermautotrophicus
1500
-
 CO2 pH 8.5, 25°C, mutant D34A/R36A, imidazole buffer Methanothermobacter thermautotrophicus
3600
-
 CO2 pH 8.5, 25°C, mutant D34E Methanothermobacter thermautotrophicus
4200
-
 CO2 pH 8.5, 25°C, mutant D34A Methanothermobacter thermautotrophicus
9100
-
 CO2 pH 8.5, 25°C, mutant D34A, imidazole buffer Methanothermobacter thermautotrophicus
11000
-
 CO2 pH 8.5, 25°C, wild-type, MOPS buffer Methanothermobacter thermautotrophicus
13000
-
 CO2 pH 8.5, 25°C, wild-type, imidazole buffer Methanothermobacter thermautotrophicus
24000
-
 CO2 pH 8.5, 25°C, wild-type Methanothermobacter thermautotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
6.4 9
-
 Methanothermobacter thermautotrophicus