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Literature summary for 4.2.1.1 extracted from
- Hydroxyurea is a carbonic anhydrase inhibitor (2003), Bioorg. Med. Chem., 11, 2241-2246.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hCA I and hCA II cDNA expressed in escherichia coli BL21 (DE3) | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanamide | - |
Homo sapiens |  |
| Hydroxyurea | - |
Homo sapiens | |
| N-(5-(aminosulfonyl)-1,3,4-thiadiazol-2-yl)acetamide | - |
Homo sapiens | |
| nitrate | - |
Homo sapiens | |
| sulfamide | - |
Homo sapiens | |
| thiocyanate | - |
Homo sapiens | |
| Urea | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.92 | - |
4-nitrophenyl acetate | pH 7.8, 25°C, hCA II | Homo sapiens | |
| 2.94 | - |
4-nitrophenyl acetate | pH 7.8, 25°C, hCA II, 25 mM hydroxyurea | Homo sapiens | |
| 15.3 | - |
4-nitrophenyl acetate | pH 7.8, 25°C, hCA I, 25 mM hydroxyurea | Homo sapiens | |
| 15.4 | - |
4-nitrophenyl acetate | pH 7.8, 25°C, hCA I | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Homo sapiens | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
r | |
| H2CO3 | - |
Homo sapiens | CO2 + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CA | - |
Homo sapiens |
| carbonic anhydrase | - |
Homo sapiens |
| hCA I | - |
Homo sapiens |
| HCA II | - |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
sulfamide | pH 7.8, 25°C, hCA I | Homo sapiens | |
| 0.082 | - |
sulfamide | pH 7.8, 25°C, hCA II | Homo sapiens | |
| 0.1 | - |
Hydroxyurea | pH 7.8, 25°C, hCA I | Homo sapiens | |
| 0.12 | - |
Hydroxyurea | pH 7.8, 25°C, hCA II | Homo sapiens | |
| 445 | - |
Urea | pH 7.8, 25°C, hCA I | Homo sapiens | |
| 460 | - |
Urea | pH 7.8, 25°C, hCA II | Homo sapiens | |
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Release 2023.1 (January 2023)
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