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Literature summary for 4.2.1.1 extracted from

  • Alber, B.E.; Colangelo, C.M.; Dong, J.; Stalhandske, C.M.V.; Baird, T.T.; Tu, C.; Fierke, C.A.; Silverman, D.N.; Scott, R.A.; Ferry, J.G.
    Kinetic and spectroscopic characterization of the gamma-carbonic anhydrase from the methanoarcheon Methanosarcina thermophila (1999), Biochemistry, 38, 13119-13128.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cam gene expressed in Escherichia coli Methanosarcina thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ isomorphously replaces zinc Methanosarcina thermophila
Zn2+
-
Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
apo-Cam and Co-Cam, gel filtration Methanosarcina thermophila
74000
-
Cam and Zn-Cam, gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Methanosarcina thermophila
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Methanosarcina thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity Cam 720 units/mg, apo-Cam 20 units/mg, Co-Cam 2020 units/mg, Zn-Cam 910 units/mg, one unit = (t0-t)/t where t0 is the time for the uncatalyzed reaction and t is the time for the catalyzed reaction Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Methanosarcina thermophila CO2 + H2O
-
r

Synonyms

Synonyms Comment Organism
gamma-carbonic anhydrase
-
Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00061
-
CO2 pH 5.5-7.5, 25°C, Zn-Cam Methanosarcina thermophila
0.00104
-
CO2 pH 5.5-7.5, 25°C, Co-Cam Methanosarcina thermophila