Cloned (Comment) | Organism |
---|---|
cam gene expressed in Escherichia coli | Methanosarcina thermophila |
Crystallization (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | isomorphously replaces zinc | Methanosarcina thermophila | |
Zn2+ | - |
Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
apo-Cam and Co-Cam, gel filtration | Methanosarcina thermophila |
74000 | - |
Cam and Zn-Cam, gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Methanosarcina thermophila | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity Cam 720 units/mg, apo-Cam 20 units/mg, Co-Cam 2020 units/mg, Zn-Cam 910 units/mg, one unit = (t0-t)/t where t0 is the time for the uncatalyzed reaction and t is the time for the catalyzed reaction | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Methanosarcina thermophila | CO2 + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
gamma-carbonic anhydrase | - |
Methanosarcina thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00061 | - |
CO2 | pH 5.5-7.5, 25°C, Zn-Cam | Methanosarcina thermophila | |
0.00104 | - |
CO2 | pH 5.5-7.5, 25°C, Co-Cam | Methanosarcina thermophila |