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Literature summary for 4.2.1.1 extracted from

  • Cronk, J.D.; O'Neill, J.W.; Cronk, M.R.; Endrizzi, J.A.; Zhang, K.Y.J.
    Cloning, crystallization and preliminary characterization of a beta-carbonic anhydrase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 1176-1179.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
CynT2 expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
CynT2, form 1, space group P4(2)212, unit-cell parameters a : b : 68.54 A, c : 85.88 A, form 2, space group P4(3)22, a : b : 81.00 A, c : 161.98 A, form 3, space group P2(1), a : 48.21, b : 140.73, c : 72.57, selenomethionine-substituted crystals, space group P4(3)22, a : b : 81.24 A, c : 162.14 A Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metalloenzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
SDS-PAGE Escherichia coli
25100
-
mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Escherichia coli
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P61517
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Escherichia coli CO2 + H2O
-
r

Synonyms

Synonyms Comment Organism
beta-carbonic anhydrase
-
Escherichia coli
carbonate hydrolase
-
Escherichia coli
CynT2
-
Escherichia coli