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Literature summary for 4.2.1.1 extracted from

  • Larsson, S.; Bjorkbacka, H.; Forsman, C.; Samuelsson, G.; Olsson, O.
    Molecular cloning and biochemical characterization of carbonic anhydrase from Populus tremula x tremuloides (1997), Plant Mol. Biol., 34, 583-592.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Populus tremula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
CO2 pH 8.8, TAPS buffer Populus tremula
9
-
CO2 pH 6.6, MES buffer Populus tremula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24800
-
8 * 24800, calculation from nucleotide sequence Populus tremula
205000
-
gel filtration Populus tremula

Organism

Organism UniProt Comment Textmining
Populus tremula
-
tremuloides
-

Purification (Commentary)

Purification (Comment) Organism
-
Populus tremula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Populus tremula H2CO3
-
?

Subunits

Subunits Comment Organism
octamer 8 * 24800, calculation from nucleotide sequence Populus tremula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2670
-
CO2 pH 6.6, MES buffer Populus tremula
3000
-
CO2 pH 8.8, TAPS buffer Populus tremula