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Literature summary for 4.2.1.1 extracted from
- The complete sequence, expression in Escherichia coli, purification and some properties of carbonic anhydrase from Neisseria gonorrhoeae (1997), Eur. J. Biochem., 244, 755-760.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Neisseria gonorrhoeae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
CO2 | - |
Neisseria gonorrhoeae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Neisseria gonorrhoeae |
| 28085 | - |
x * 28085, a 26-residue signal peptide is cleaved off by the Escherichia coli processing machinery, thus the recombinant enzyme has a MW of 26314 Da, calculation from nucleotide sequence | Neisseria gonorrhoeae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria gonorrhoeae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | a 26-residue signal peptide is cleaved off by the E. coli processing machinery | Neisseria gonorrhoeae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neisseria gonorrhoeae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | - |
Neisseria gonorrhoeae | H2CO3 | - |
? | |
| additional information | esterase activity with p-nitrophenyl acetate | Neisseria gonorrhoeae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Neisseria gonorrhoeae |
| ? | x * 28085, a 26-residue signal peptide is cleaved off by the Escherichia coli processing machinery, thus the recombinant enzyme has a MW of 26314 Da, calculation from nucleotide sequence | Neisseria gonorrhoeae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18300 | - |
CO2 | - |
Neisseria gonorrhoeae | |
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