Literature summary for 4.2.1.1 extracted from

Kisiel, W.; Graf, G.
Purification and characterization of carbonic anhydrase from Pisum sativum (1972), Phytochemistry, 11, 113-117.

No PubMed abstract available

Search for more literature for 4.2.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
acetazolamide	-	Pisum sativum
HCO3-	substrate inhibition above 0.1 M	Pisum sativum
Hg2+	-	Pisum sativum
o-Iodosobenzoate	-	Pisum sativum
PCMB	0.25 mM, complete inhibition after 10 min	Pisum sativum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
30	-	HCO3-	-	Pisum sativum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	contains 6 mol of zinc per mol of enzyme	Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	6 * 30000, SDS-PAGE	Pisum sativum
194000	-	gel filtration, meniscus depletion equilibrium sedimentation, analytical ultracentrifugation	Pisum sativum

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pisum sativum

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Pisum sativum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pisum sativum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Pisum sativum	H2CO3	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 30000, SDS-PAGE	Pisum sativum

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Release 2023.1 (January 2023)