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Literature summary for 4.2.1.1 extracted from

  • Jones, G.L.; Shaw, D.C.
    Purification, properties, partial sequence and evolutionary relationships of marsupial erythrocyte carbonic anhydrase (1982), Biochim. Biophys. Acta, 709, 284-303.
    View publication on PubMed

General Stability

General Stability Organism
dialysis against pyridine dicarboxylate, at 15°C, 98% loss of activity after 24 h Macropus eugenii desmarest

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
Macropus eugenii desmarest
p-hydroxymercuribenzoate
-
Macropus eugenii desmarest
PCMB reversible with 5 mM dithiothreitol Macropus eugenii desmarest

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.67
-
CO2
-
Macropus eugenii desmarest

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ when added back to the apoenzyme in a 1:1 molar ratio or greater, the activity immediately returns to control level Macropus eugenii desmarest
Ni2+ slight activation of the apoenzyme Macropus eugenii desmarest
Zinc Zn2+, when added back to the apoenzyme in a 1:1 molar ratio or greater, the activity immediately returns to control level Macropus eugenii desmarest

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28200
-
1 * 28200, carbonic anhydrase A, SDS-PAGE Macropus eugenii desmarest
30700
-
gel filtration Macropus eugenii desmarest

Organism

Organism UniProt Comment Textmining
Macropus eugenii desmarest
-
tammar wallaby
-

Purification (Commentary)

Purification (Comment) Organism
-
Macropus eugenii desmarest

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Macropus eugenii desmarest
-

Storage Stability

Storage Stability Organism
4°C, stable for up to 1 year Macropus eugenii desmarest

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Macropus eugenii desmarest H2CO3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28200, carbonic anhydrase A, SDS-PAGE Macropus eugenii desmarest

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
t1/2: more than 120 min Macropus eugenii desmarest