Literature summary for 4.1.2.8 extracted from

Dunn, M.F.; Niks, D.; Ngo, H.; Barends, T.R.; Schlichting, I.
Tryptophan synthase: the workings of a channeling nanomachine (2008), Trends Biochem. Sci., 33, 254-264.

Search for more literature for 4.1.2.8

Protein Variants

Protein Variants	Comment	Organism
D60N	site-directed mutagenesis, substitution of asparagine for aspartic acid in the alphaAsp60Asn variant. alphaAsp60 has an essential role in stabilizing charge development on the IGP indole ring during C-C bond scission	Salmonella enterica subsp. enterica serovar Typhimurium
T183V	site-directed mutagenesis, substitution of valine for threonine in the alphaThr183Val variant, resulting in impaired catalysis at the alpha-site and loss of allosteric communication	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-dihydroxy-3-[(2-hydroxyphenyl)amino] propyl phosphate	competetive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
2-([[4-(trifluoromethoxy)phenyl]sulfonyl]amino) ethyl phosphate	competetive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
3-(1H-indol-3-yl)propyl phosphate	competetive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
3-(2,3-dihydro-1H-indol-3-yl)glycerol phosphate	competetive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
3-acetyl-L-aspartic acid	inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen	Salmonella enterica subsp. enterica serovar Typhimurium
4-[(2-aminophenyl)sulfonyl]butyl phosphate	competetive inhibitor	Salmonella enterica subsp. enterica serovar Typhimurium
indole 3-acetylglycine	inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
indole-3-glycerol phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	indole + D-glyceraldehyde 3-phosphate	-	?

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Release 2023.1 (January 2023)