Cloned (Comment) | Organism |
---|---|
into the vectorS pET28a, pEZS-NL and pPCVE35E | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.458 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Zea mays | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | Zea mays | - |
indole + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | B2Y0K4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein by His-tag affinity purification via Ni-NTA agarose, plant protein by anion exchange and gel permeation chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays | indole + D-glyceraldehyde 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tryptophan synthase alpha subunit | - |
Zea mays |
ZmTSA | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
tryptophan synthase assay | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
tryptophan synthase assay | Zea mays |