Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.13 extracted from

  • Stolzenberger, J.; Lindner, S.N.; Wendisch, V.F.
    The methylotrophic Bacillus methanolicus MGA3 possesses two distinct fructose 1,6-bisphosphate aldolases (2013), Microbiology, 159, 1770-1781.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus methanolicus

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM, 47% residual activity; 1 mM, 58% residual activity Bacillus methanolicus
ATP 1 mM, 34% residual activity; 1 mM, 39% residual activity Bacillus methanolicus
Cd2+ 1 mM, no residual activity; 1 mM, no residual activity Bacillus methanolicus
Cu2+ 1 mM, 3% residual activity; 1 mM, 5% residual activity Bacillus methanolicus
D-fructose 1,6-bisphosphate substrate inhibition; substrate inhibition Bacillus methanolicus
EDTA 1 mM, no residual activity; 1 mM, no residual activity Bacillus methanolicus
Fe2+ 1 mM, 15% residual activity; 1 mM, 18% residual activity Bacillus methanolicus
Zn2+ 1 mM, 15% residual activity; 1 mM, 8% residual activity Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
D-fructose 1,6-bisphosphate pH 7.6, 50°C Bacillus methanolicus
0.25
-
D-glyceraldehyde 3-phosphate pH 7.8, 55°C Bacillus methanolicus
0.58
-
D-glyceraldehyde 3-phosphate pH 7.6, 50°C Bacillus methanolicus
1
-
glycerone phosphate pH 7.6, 50°C Bacillus methanolicus
2
-
D-fructose 1,6-bisphosphate pH 7.8, 55°C Bacillus methanolicus
2
-
glycerone phosphate pH 7.8, 55°C Bacillus methanolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 128% of initial activity activity Bacillus methanolicus
Co2+ 1 mM, 131% of initial activity activity Bacillus methanolicus
Mn2+ 1 mM, 148% of inital activity Bacillus methanolicus
Mn2+ 1 mM, 156% of inital activity Bacillus methanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30704
-
4 * 30704, calculated Bacillus methanolicus
124000
-
gel filtration Bacillus methanolicus
129000
-
gel filtration Bacillus methanolicus

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
-
-
Bacillus methanolicus I3EBM6 chromosomally located isoform FbaC
-
Bacillus methanolicus MGA3
-
-
-
Bacillus methanolicus MGA3 I3EBM6 chromosomally located isoform FbaC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Bacillus methanolicus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r
D-fructose 1,6-bisphosphate
-
Bacillus methanolicus MGA3 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r
Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
Bacillus methanolicus D-Fructose 1,6-bisphosphate
-
r
Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
Bacillus methanolicus MGA3 D-Fructose 1,6-bisphosphate
-
r
additional information no substrate: fructose 1-phosphate, fructose 6-phosphate Bacillus methanolicus ?
-
?
additional information no substrate: fructose 1-phosphate, fructose 6-phosphate. No additional phosphates activity is observed Bacillus methanolicus ?
-
?
additional information no substrate: fructose 1-phosphate, fructose 6-phosphate. No additional phosphates activity is observed Bacillus methanolicus MGA3 ?
-
?
additional information no substrate: fructose 1-phosphate, fructose 6-phosphate Bacillus methanolicus MGA3 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 30704, calculated Bacillus methanolicus
tetramer calculated Bacillus methanolicus

Synonyms

Synonyms Comment Organism
Fba2
-
Bacillus methanolicus
FbaC
-
Bacillus methanolicus
FbaP
-
Bacillus methanolicus
MGA3_01355
-
Bacillus methanolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus methanolicus
55
-
-
Bacillus methanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Bacillus methanolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
glycerone phosphate pH 7.6, 50°C Bacillus methanolicus
0.56
-
D-glyceraldehyde 3-phosphate pH 7.6, 50°C Bacillus methanolicus
1.6
-
D-fructose 1,6-bisphosphate pH 7.8, 55°C Bacillus methanolicus
5.1
-
D-fructose 1,6-bisphosphate pH 7.6, 50°C Bacillus methanolicus
8
-
glycerone phosphate pH 7.8, 55°C Bacillus methanolicus
12.6
-
D-glyceraldehyde 3-phosphate pH 7.8, 55°C Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Bacillus methanolicus
7.8
-
-
Bacillus methanolicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Bacillus methanolicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2 5 D-fructose 1,6-bisphosphate pH 7.8, 55°C Bacillus methanolicus
22
-
D-fructose 1,6-bisphosphate pH 7.6, 50°C Bacillus methanolicus

General Information

General Information Comment Organism
physiological function isoform FbaC acts as major aldolase in glycolysis, whereas isoform FbaP acts as major aldolase in gluconeogenesis in Bacillus methanolicus. The aldolase-negative Corynebacterium glutamicum mutant Dfda can be complemented by the FbaC gene Bacillus methanolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
glycerone phosphate pH 7.6, 50°C Bacillus methanolicus
0.8
-
D-fructose 1,6-bisphosphate pH 7.8, 55°C Bacillus methanolicus
1.4
-
D-glyceraldehyde 3-phosphate pH 7.6, 50°C Bacillus methanolicus
4
-
glycerone phosphate pH 7.8, 55°C Bacillus methanolicus
31.3
-
D-fructose 1,6-bisphosphate pH 7.6, 50°C Bacillus methanolicus
50.4
-
D-glyceraldehyde 3-phosphate pH 7.8, 55°C Bacillus methanolicus