Literature summary for 4.1.2.13 extracted from

Tunio, S.A.; Oldfield, N.J.; Berry, A.; Alaaldeen, D.A.; Wooldridge, K.G.; Turner, D.P.
The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: surface localization and role in host cell adhesion (2010), Mol. Microbiol., 76, 605-615.

Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment)	Organism
TOP10F' and BL21(DE3) pLysS are used for the expression of 6x histidine-tagged recombinant enzyme encoded by plasmid pSAT-FBA	Neisseria meningitidis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	D-fructose 1,6-bisphosphate	at 30°C in 50 mM Tris-HCl, 0.1 M potassium acetate buffer (pH 8.0)	Neisseria meningitidis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Neisseria meningitidis	5737	-
outer membrane	-	Neisseria meningitidis	19867	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	SDS-PAGE	Neisseria meningitidis

Organism

Organism	UniProt	Comment	Textmining
Neisseria meningitidis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisPur cobalt resin column chromatography	Neisseria meningitidis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Neisseria meningitidis	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
FBA	-	Neisseria meningitidis
fructose-1,6-bisphosphate aldolase	-	Neisseria meningitidis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	D-fructose 1,6-bisphosphate	at 30°C in 50 mM Tris-HCl, 0.1 M potassium acetate buffer (pH 8.0)	Neisseria meningitidis

General Information

General Information	Comment	Organism
physiological function	FBA is required for optimal adhesion of meningococci to human cells	Neisseria meningitidis

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Release 2023.1 (January 2023)