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Literature summary for 4.1.2.13 extracted from

  • Kakita, H.; Kamishima, H.; Inouye, K.
    Purification and some properties of a D-fructose-1,6-bisphosphate aldolase from the red alga, Gracilaria chorda Holmes (2006), Bot. Mar., 49, 174-181.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 38% residual activity Gracilariopsis chorda
EDTA 0.025 mM and above, no residual activity Gracilariopsis chorda
glutathione 10 mM, 62% residual activity Gracilariopsis chorda
iodoacetamide 76% residual activity at 10 mM Gracilariopsis chorda
L-cysteine 10 mM, complete inhibition Gracilariopsis chorda

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the enzyme is activated up to 5.5fold by the addition of 500 mM KCl Gracilariopsis chorda
additional information 1 mM Fe2+, Ca2+, or Mg2+ fail to recover the activity lost by 0.5 mM EDTA application and 0.5 mM cysteine and 1 mM 2-mercaptoethanol have almost no effect on the enzyme activity Gracilariopsis chorda
Zn2+ the EDTA-inhibited activity can be restored to 109% of the original activity by the addition of 1 mM ZnSO4 Gracilariopsis chorda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
SDS-PAGE Gracilariopsis chorda
45000
-
SDS-PAGE Gracilariopsis chorda

Organism

Organism UniProt Comment Textmining
Gracilariopsis chorda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Toyopearl 650 S column chromatography, and TSKgel Phenyl 5PW column chromatography Gracilariopsis chorda

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.123
-
after 1fold purification, at 25°C Gracilariopsis chorda
10.7
-
after 87fold purification, at 25°C Gracilariopsis chorda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Gracilariopsis chorda glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
D-fructose-1,6-bisphosphate aldolase
-
Gracilariopsis chorda
FBP aldolase
-
Gracilariopsis chorda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Gracilariopsis chorda