Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 38% residual activity | Gracilariopsis chorda | |
EDTA | 0.025 mM and above, no residual activity | Gracilariopsis chorda | |
glutathione | 10 mM, 62% residual activity | Gracilariopsis chorda | |
iodoacetamide | 76% residual activity at 10 mM | Gracilariopsis chorda | |
L-cysteine | 10 mM, complete inhibition | Gracilariopsis chorda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the enzyme is activated up to 5.5fold by the addition of 500 mM KCl | Gracilariopsis chorda | |
additional information | 1 mM Fe2+, Ca2+, or Mg2+ fail to recover the activity lost by 0.5 mM EDTA application and 0.5 mM cysteine and 1 mM 2-mercaptoethanol have almost no effect on the enzyme activity | Gracilariopsis chorda | |
Zn2+ | the EDTA-inhibited activity can be restored to 109% of the original activity by the addition of 1 mM ZnSO4 | Gracilariopsis chorda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
SDS-PAGE | Gracilariopsis chorda |
45000 | - |
SDS-PAGE | Gracilariopsis chorda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gracilariopsis chorda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Toyopearl 650 S column chromatography, and TSKgel Phenyl 5PW column chromatography | Gracilariopsis chorda |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.123 | - |
after 1fold purification, at 25°C | Gracilariopsis chorda |
10.7 | - |
after 87fold purification, at 25°C | Gracilariopsis chorda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Gracilariopsis chorda | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-fructose-1,6-bisphosphate aldolase | - |
Gracilariopsis chorda |
FBP aldolase | - |
Gracilariopsis chorda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Gracilariopsis chorda |