Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D109A | very low catalytic activity | Escherichia coli |
D144A | increased Km | Escherichia coli |
D288A | increased Km | Escherichia coli |
D290A | increased Km | Escherichia coli |
D329A | increased Km | Escherichia coli |
N286A | increased Km, decreased kcat | Escherichia coli |
N286D | increased Km, severely decreased kcat | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | - |
Escherichia coli | |
glyceraldehyde 3-phosphate | non-competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-fructose 1,6-bisphosphate | 30°C, D109A mutant | Escherichia coli | |
0.19 | - |
D-fructose 1,6-bisphosphate | 30°C, wild-type enzyme | Escherichia coli | |
0.37 | - |
D-fructose 1,6-bisphosphate | 30°C, N286D mutant | Escherichia coli | |
0.77 | - |
D-fructose 1,6-bisphosphate | 30°C, D290A mutant | Escherichia coli | |
0.92 | - |
D-fructose 1,6-bisphosphate | 30°C, D329A mutant | Escherichia coli | |
0.94 | - |
D-fructose 1,6-bisphosphate | 30°C, D144A mutant | Escherichia coli | |
1 | - |
D-fructose 1,6-bisphosphate | 30°C, D288A mutant | Escherichia coli | |
1.07 | - |
D-fructose 1,6-bisphosphate | 30°C, N286A mutant | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Escherichia coli | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AB71 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate | mechanism of class II aldolase | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Escherichia coli | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
D-fructose 1,6-bisphosphate | 30°C, N286D mutant | Escherichia coli | |
0.0028 | - |
D-fructose 1,6-bisphosphate | 30°C, D109A mutant | Escherichia coli | |
0.19 | - |
D-fructose 1,6-bisphosphate | 30°C, N286A mutant | Escherichia coli | |
2 | - |
D-fructose 1,6-bisphosphate | 30°C, D144A mutant | Escherichia coli | |
2.2 | - |
D-fructose 1,6-bisphosphate | 30°C, D288A mutant | Escherichia coli | |
7 | - |
D-fructose 1,6-bisphosphate | 30°C, D290A mutant | Escherichia coli | |
8.2 | - |
D-fructose 1,6-bisphosphate | 30°C, wild-type enzyme | Escherichia coli | |
12.3 | - |
D-fructose 1,6-bisphosphate | 30°C, D329A mutant | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
dihydroxyacetone phosphate | 30°C, wild-type enzyme | Escherichia coli | |
1.7 | - |
dihydroxyacetone phosphate | 30°C, N268A mutant | Escherichia coli |