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Literature summary for 4.1.2.13 extracted from

  • Willard, J.M.; Gibbs, M.
    Fructose-diphosphate aldolase from blue-green algae (1975), Methods Enzymol., 42C, 228-234.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Cys maximal activity only when both 8 mM Cys and 0.1 mM Fe2+ are present. Fe3+ or Mn2+ can replace Fe2+ Synechococcus elongatus PCC 7942 = FACHB-805

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
D-fructose 1,6-bisphosphate
-
Synechococcus elongatus PCC 7942 = FACHB-805
10
-
sedoheptulose 1,7-diphosphate
-
Synechococcus elongatus PCC 7942 = FACHB-805

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ maximal activity only when both 8 mM Cys and 0.1 mM Fe2+ are present. Fe3+ or Mn2+ can replace Fe2+ Synechococcus elongatus PCC 7942 = FACHB-805
Fe3+ maximal activity only when both 8 mM Cys and 0.1 mM Fe2+ are present. Fe3+ or Mn2+ can replace Fe2+ Synechococcus elongatus PCC 7942 = FACHB-805
K+ no effect Synechococcus elongatus PCC 7942 = FACHB-805
Mn2+ maximal activity only when both 8 mM Cys and 0.1 mM Fe2+ are present. Fe3+ or Mn2+ can replace Fe2+ Synechococcus elongatus PCC 7942 = FACHB-805

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137000
-
sucrose density gradient centrifugation Synechococcus elongatus PCC 7942 = FACHB-805

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Synechococcus elongatus PCC 7942 = FACHB-805

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
128
-
-
Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Synechococcus elongatus PCC 7942 = FACHB-805 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
sedoheptulose 1,7-bisphosphate at 59% of the activity with fructose 1,6-diphosphate Synechococcus elongatus PCC 7942 = FACHB-805 glycerone phosphate + D-erythrose 4-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Synechococcus elongatus PCC 7942 = FACHB-805