Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | design of a surface-modified variant incorporating 11 changes in the amino acids on the protein surface to stabilze the protein at acidic pH. Mutant shows a broadened pH optimum towards the acidic range, along with enhancement of activity by up to twofold and significantly increased pH- and thermostabilities. The effect is probably due to a shift of the isoelectic point from pH 5.1 to 4.8. Mutant is applicable in aqueous/organic two-phase systems | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LFT6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.2 | - |
wild-type, 25°C, pH 4.75 | Arabidopsis thaliana |
23.6 | - |
surface-modified mutant, 25°C, pH 4.5 | Arabidopsis thaliana |
133.6 | - |
wild-type, 25°C, pH 6.0 | Arabidopsis thaliana |
227 | - |
surface-modified mutant, 25°C, pH 5.75 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelonitrile | - |
Arabidopsis thaliana | cyanide + benzaldehyde | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
pH 5.0, wild-type, half-life 18 min, surface-modified mutant, half-life 137 min | Arabidopsis thaliana |
10 | - |
pH 5.0, wild-type, half-life 23.6 min, surface-modified mutant, half-life 136 min | Arabidopsis thaliana |
20 | - |
pH 5.0, wild-type, half-life 9.6 min, surface-modified mutant, half-life 134 min | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.75 | - |
surface-modified mutant | Arabidopsis thaliana |
6 | - |
wild-type | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
surface-modified mutant, less than 10% of maximum activity | Arabidopsis thaliana |
4.75 | - |
wild-type, less than 10% of maximum activity | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
20°C, wild-type, half-life 0.16 h, surface-modified mutant, half-life 2.23 h | Arabidopsis thaliana |
6 | - |
20°C, wild-type, half-life 60 h, surface-modified mutant, half-life 52.2 h | Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | isoelectric focusing, wild-type | - |
5.1 |