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Literature summary for 4.1.2.10 extracted from

  • Yildirim, D.; Tuekel, S.S.; Alagoez, D.
    Crosslinked enzyme aggregates of hydroxynitrile lyase partially purified from Prunus dulcis seeds and its application for the synthesis of enantiopure cyanohydrins (2014), Biotechnol. Prog., 30, 818-827.
    View publication on PubMed

Application

Application Comment Organism
synthesis purification of enzyme as crosslinked enzyme aggregates and application for synthesis of enantiopure cyanohydrins Prunus dulcis

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partly, as crosslinked enzyme aggregates. Optimal conditions are initial glutaraldehyde concentration of 25% w/v, ammonium sulfate saturation concentration of 43% w/v, and crosslinking time of 18 h Prunus dulcis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Prunus dulcis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobenzaldehyde + HCN
-
Prunus dulcis (R)-2-chloromandelonitrile after 96 h, 100% yield, 21% enantiomeric excess ?
3,4-dihydroxybenzaldehyde + HCN
-
Prunus dulcis (R)-3,4-dihydroxymandelonitrile after 96 h, 100% yield, 99% enantiomeric excess ?
3-phenylpropionaldehyde + HCN
-
Prunus dulcis (R)-2-hydroxy-4-phenylbutyronitrile after 96 h, 83% yield, 91% enantiomeric excess ?
4-bromobenzaldehyde + HCN
-
Prunus dulcis (R)-4-bromomandelonitrile after 96 h, 100% yield, 99% enantiomeric excess ?
4-fluorobenzaldehyde + HCN
-
Prunus dulcis (R)-4-fluoromandelonitrile after 96 h, 100% yield, 72% enantiomeric excess ?
4-nitrobenzaldehyde + HCN
-
Prunus dulcis (R)-4-nitromandelonitrile after 96 h, 100% yield, 14% enantiomeric excess ?
benzaldehyde + HCN
-
Prunus dulcis (R)-mandelonitrile after 96 h, 100% yield, 99% enantiomeric excess ?