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Literature summary for 4.1.2.10 extracted from

  • Fuhshuku, K.; Asano, Y.
    Synthesis of (R)-beta-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system (2011), J. Biotechnol., 153, 153-159.
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of (R)-beta-nitro alcohols using nitromethane as donor. Highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Organic Solvent Stability

Organic Solvent Comment Organism
butyl acetate synthesis of (R)-beta-nitro alcohols, highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q9LFT6
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
Arabidopsis thaliana cyanide + benzaldehyde
-
?
2-chlorobenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(2-chlorophenyl)-2-nitroethanol 34% yield after 2 h ?
2-methoxybenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(2-methoxyphenyl)-2-nitroethanol 13% yield after 2 h ?
2-methylbenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(2-methylphenyl)-2-nitroethanol 12% yield after 2 h ?
3-(2-naphthyl)benzaldehyde + nitromethane
-
Arabidopsis thaliana (1R)-1-[3-(naphthalen-2-yl)phenyl]-2-nitroethanol 7% yield after 2 h ?
3-chlorobenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(3-chlorophenyl)-2-nitroethanol 17% yield after 2 h ?
3-methoxybenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(3-methoxyphenyl)-2-nitroethanol 17% yield after 2 h ?
3-methylbenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(3-methylphenyl)-2-nitroethanol 12% yield after 2 h ?
4-bromobenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(4-bromophenyl)-2-nitroethanol 20% yield after 2 h ?
4-chlorobenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(4-chlorophenyl)-2-nitroethanol 9% yield after 2 h ?
4-fluorobenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(4-fluorophenyl)-2-nitroethanol 9% yield after 2 h ?
4-methoxybenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(4-methoxyphenyl)-2-nitroethanol 2% yield after 2 h ?
4-methylbenzaldehyde + nitromethane
-
Arabidopsis thaliana 1-(4-methylphenyl)-2-nitroethanol 11% yield after 2 h ?
benzaldehyde + nitromethane
-
Arabidopsis thaliana (R)-2-nitro-1-phenylethanol 30% yield after 2 h ?
nitromethane + 2-chlorobenzaldehyde
-
Arabidopsis thaliana (1R)-1-(2-chlorophenyl)-2-nitroethanol 34% yield, 68% enantiomeric excess ?
nitromethane + 3-methoxybenzaldehyde
-
Arabidopsis thaliana (1R)-1-(3-methoxyphenyl)-2-nitroethanol 17% yield, 91% enantiomeric excess ?
nitromethane + 4-fluorobenzaldehyde
-
Arabidopsis thaliana (1R)-1-(4-fluorophenyl)-2-nitroethanol 20% yield, 81% enantiomeric excess ?
nitromethane + benzaldehyde 30% yield, 91% enantiomeric excess Arabidopsis thaliana (1R)-2-nitro-1-phenylethanol
-
?

Synonyms

Synonyms Comment Organism
R-HNL
-
Arabidopsis thaliana
R-selective hydroxynitrile lyase
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Arabidopsis thaliana