Application | Comment | Organism |
---|---|---|
synthesis | synthesis of (R)-beta-nitro alcohols using nitromethane as donor. Highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Organic Solvent | Comment | Organism |
---|---|---|
butyl acetate | synthesis of (R)-beta-nitro alcohols, highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9LFT6 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni Sepharose column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelonitrile | - |
Arabidopsis thaliana | cyanide + benzaldehyde | - |
? | |
2-chlorobenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(2-chlorophenyl)-2-nitroethanol | 34% yield after 2 h | ? | |
2-methoxybenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(2-methoxyphenyl)-2-nitroethanol | 13% yield after 2 h | ? | |
2-methylbenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(2-methylphenyl)-2-nitroethanol | 12% yield after 2 h | ? | |
3-(2-naphthyl)benzaldehyde + nitromethane | - |
Arabidopsis thaliana | (1R)-1-[3-(naphthalen-2-yl)phenyl]-2-nitroethanol | 7% yield after 2 h | ? | |
3-chlorobenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(3-chlorophenyl)-2-nitroethanol | 17% yield after 2 h | ? | |
3-methoxybenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(3-methoxyphenyl)-2-nitroethanol | 17% yield after 2 h | ? | |
3-methylbenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(3-methylphenyl)-2-nitroethanol | 12% yield after 2 h | ? | |
4-bromobenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(4-bromophenyl)-2-nitroethanol | 20% yield after 2 h | ? | |
4-chlorobenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(4-chlorophenyl)-2-nitroethanol | 9% yield after 2 h | ? | |
4-fluorobenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(4-fluorophenyl)-2-nitroethanol | 9% yield after 2 h | ? | |
4-methoxybenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(4-methoxyphenyl)-2-nitroethanol | 2% yield after 2 h | ? | |
4-methylbenzaldehyde + nitromethane | - |
Arabidopsis thaliana | 1-(4-methylphenyl)-2-nitroethanol | 11% yield after 2 h | ? | |
benzaldehyde + nitromethane | - |
Arabidopsis thaliana | (R)-2-nitro-1-phenylethanol | 30% yield after 2 h | ? | |
nitromethane + 2-chlorobenzaldehyde | - |
Arabidopsis thaliana | (1R)-1-(2-chlorophenyl)-2-nitroethanol | 34% yield, 68% enantiomeric excess | ? | |
nitromethane + 3-methoxybenzaldehyde | - |
Arabidopsis thaliana | (1R)-1-(3-methoxyphenyl)-2-nitroethanol | 17% yield, 91% enantiomeric excess | ? | |
nitromethane + 4-fluorobenzaldehyde | - |
Arabidopsis thaliana | (1R)-1-(4-fluorophenyl)-2-nitroethanol | 20% yield, 81% enantiomeric excess | ? | |
nitromethane + benzaldehyde | 30% yield, 91% enantiomeric excess | Arabidopsis thaliana | (1R)-2-nitro-1-phenylethanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
R-HNL | - |
Arabidopsis thaliana |
R-selective hydroxynitrile lyase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |