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Literature summary for 4.1.1.9 extracted from

  • Zhou, D.; Yuen, P.; Chu, D.; Thon, V.; McConnell, S.; Brown, S.; Tsang, A.; Pena, M.; Russell, A.; Cheng, J.F.; Nadzan, A.M.; Barbosa, M.S.; Dyck, J.R.B.; Lopaschuk, G.D.; Yang, G.
    Expression, purification, and characterization of human malonyl-CoA decarboxylase (2004), Protein Expr. Purif., 34, 261-269.
    View publication on PubMed
Search for more literature for 4.1.1.9

Application

Application Comment Organism
medicine enzyme deficiency is characterized by malonic aciduria, developmental delay, seizure disorder, and mental retardation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
from heart, overexpression of full-length and N-terminal truncated hMCD in Escherichia coli BL21 (DE3), sequencing Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Homo sapiens

General Stability

General Stability Organism
the N-terminal region of hMCD plays a role in the folding process and conformation stability of enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33 0.52 malonyl-CoA full-length and N-terminal truncated apo-hMCD Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
additional information full-length MCD contains a putative, N-terminal 39 amino acid mitochondria targeting sequence and a peroxisomal tageting sequence Homo sapiens
-
-
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement of divalent metal ions Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, about, wild-type apo-hMCD, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Malonyl-CoA Homo sapiens plays a critical role in fatty-acid metabolism, precursor hMCD plays a role in the regulation of the cytosolic malonyl-CoA level in cells Acetyl-CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification full-length MCD contains a putative, N-terminal 39 amino acid mitochondria targeting sequence and a peroxisomal tageting sequence Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length and N-terminal truncated apoenzymes Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Malonyl-CoA
-
 Homo sapiens Acetyl-CoA + CO2
-
 ?
Malonyl-CoA plays a critical role in fatty-acid metabolism, precursor hMCD plays a role in the regulation of the cytosolic malonyl-CoA level in cells Homo sapiens Acetyl-CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, about, wild-type apo-hMCD, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hMCD
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13 28 malonyl-CoA full-length and N-terminal truncated apo-hMCD Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens theoretical pI, N-terminal truncated apo-hMCD
-
 8
Homo sapiens theoretical pI, wild-type hMCD
-
 9.1