Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizobium leguminosarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 1 mM, 16% inhibition | Rhizobium leguminosarum | |
N-ethylmaleimide | 1 mM, 47% inhibition | Rhizobium leguminosarum | |
p-chloromercuribenzoate | 0.1 mM, 95.5% inhibition | Rhizobium leguminosarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
malonyl-CoA | - |
Rhizobium leguminosarum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rhizobium leguminosarum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence | Rhizobium leguminosarum |
53000 | - |
4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence | Rhizobium leguminosarum |
208000 | - |
field flow fractionation | Rhizobium leguminosarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Malonyl-CoA | Rhizobium leguminosarum | malonate metabolism | Acetyl-CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rhizobium leguminosarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhizobium leguminosarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Malonyl-CoA | primary substrate, higher specific activity than with methylmalonyl-CoA as substrate, Cys-214 is located at or near the active site and plays a critical role in catalysis | Rhizobium leguminosarum | Acetyl-CoA + CO2 | - |
? | |
Malonyl-CoA | malonate metabolism | Rhizobium leguminosarum | Acetyl-CoA + CO2 | - |
? | |
methylmalonyl-CoA | specific activity is 0.1% that of malonyl-CoA decarboxylation | Rhizobium leguminosarum | propionyl-CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 53000, SDS-PAGE, 4 * 51000, calculated from the amino acid sequence | Rhizobium leguminosarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhizobium leguminosarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhizobium leguminosarum |