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Literature summary for 4.1.1.78 extracted from
- Enzymatic utilization of acetylenic compounds. I. An enzyme converting acetylenedicarboxylic acid to pyruvate (1958), J. Biol. Chem., 233, 706-711.
General Stability
| General Stability | Organism |
|---|---|
| loss of stabililty by freezing and thawing | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al(NO3)3 | - |
Pseudomonas sp. |  |
| BaCl2 | - |
Pseudomonas sp. | |
| CN- | - |
Pseudomonas sp. | |
| CoCl2 | - |
Pseudomonas sp. | |
| EDTA | - |
Pseudomonas sp. | |
| Fe(NO3)3 | - |
Pseudomonas sp. | |
| HgCl2 | - |
Pseudomonas sp. | |
| NiCl2 | - |
Pseudomonas sp. | |
| p-chloromercuribenzoate | - |
Pseudomonas sp. | |
| ZnSO4 | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
acetylene dicarboxylate | 23°C, pH 7.3 | Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene dicarboxylate | Pseudomonas sp. | - |
pyruvate + CO2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Clostridium cylindrosporum | - |
- |
- |
| no activity in Clostridium kluyveri | - |
- |
- |
| no activity in Escherichia coli | - |
- |
- |
| no activity in Pseudomonas fluorescens | - |
- |
- |
| no activity in Rattus norvegicus | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 463 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.005 mM 2-mercaptoethanol, 10 days, less than 10% loss of activity | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene dicarboxylate | - |
Pseudomonas sp. | pyruvate + CO2 | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
enzyme assay at | Pseudomonas sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.8 | - |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Pseudomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
highest stability at 3°C for 17 h | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Pseudomonas sp. |
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Release 2023.1 (January 2023)
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