Protein Variants | Comment | Organism |
---|---|---|
E210Q | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 6fold lower than wild-type value | Saccharolobus solfataricus |
E51Q | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 28fold lower than wild-type value | Saccharolobus solfataricus |
K53Q | low activity, kcat is 570fold lower than wild-type value | Saccharolobus solfataricus |
K53R | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 500fold lower than wild-type value | Saccharolobus solfataricus |
R182A | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 39fold lower than wild-type value | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E51Q | Saccharolobus solfataricus | |
0.000044 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
0.000386 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E210Q | Saccharolobus solfataricus | |
0.00106 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme R182A | Saccharolobus solfataricus | |
0.00244 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme K53R | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q06121 | - |
- |
Saccharolobus solfataricus P2 | Q06121 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Lys53 acts as the general acid in the dehydration step of the reaction | Saccharolobus solfataricus | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Lys53 acts as the general acid in the dehydration step of the reaction | Saccharolobus solfataricus P2 | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme K53Q | Saccharolobus solfataricus | |
0.007 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E51Q | Saccharolobus solfataricus | |
0.06 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme K53R | Saccharolobus solfataricus | |
0.41 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme R182A | Saccharolobus solfataricus | |
0.67 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
0.97 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E210Q | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme K53R | Saccharolobus solfataricus | |
380 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme R182A | Saccharolobus solfataricus | |
540 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E51Q | Saccharolobus solfataricus | |
2500 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, mutant enzyme E210Q | Saccharolobus solfataricus | |
15230 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 75°C, wild-type enzyme | Saccharolobus solfataricus |