Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.39 extracted from

  • Ishikawa, C.; Hatanaka, T.; Misoo, S.; Miyake, C.; Fukayama, H.
    Functional incorporation of Sorghum small subunit increases the catalytic turnover rate of Rubisco in transgenic rice (2011), Plant Physiol., 156, 1603-1611.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
RbcS is expressed in Oryza sativa cultivar Nipponbare Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0217
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS10, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
0.0224
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS16, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
0.024
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS5, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
0.0415
-
CO2 wild type enzyme, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
variant Tentaka
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
Sorghum bicolor 3-phospho-D-glycerate
-
?

Synonyms

Synonyms Comment Organism
rbcL Rubisco large subunit Sorghum bicolor
rbcS Rubisco small subunit Sorghum bicolor
Rubisco
-
Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.22
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS16, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
2.48
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS5, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
2.52
-
CO2 recombinant enzyme expressed in Oryza sativa transgenic line SS10, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor
4.09
-
CO2 wild type enzyme, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C Sorghum bicolor

Expression

Organism Comment Expression
Sorghum bicolor the overexpression of sorghum RbcS does not down-regulate the expression of RbcS gene family and stimulates the expression of RbcL in rice. In particular, the Rubisco content in SS5, a high expressing line, is 24% higher than that in nontransgenic rice up