Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | stimulates the activation of Rubisco, all three phosphate-binding sites are involved in stimulation of Rubisco activation | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
G404A | while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is not reduced, the mutant shows a marked reductions in kcat compared to the wild type enzyme | Synechocystis sp. |
T65A | while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is reduced 40-50%, the mutant shows a marked reductions in kcat compared to the wild type enzyme | Synechocystis sp. |
T65S | while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is reduced 40-50%, the mutant shows a marked reductions in kcat compared to the wild type enzyme | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | inhibits the catalysis of Rubisco, only the 5P-site is involved in inhibition of catalysis | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0463 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.0473 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.146 | - |
D-ribulose 1,5-bisphosphate | wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.193 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.258 | - |
CO2 | mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.268 | - |
CO2 | wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.283 | - |
CO2 | mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
0.296 | - |
CO2 | mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme is activated for 30 min by 0.28 mM CO2 and 10 mM MgCl2 at 30°C | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by ammonium sulfate precipitation and Sephadex G-100 gel filtration | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Synechocystis sp. | 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Synechocystis sp. |
Rubisco | - |
Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
1.2 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
2.2 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
14.28 | - |
D-ribulose 1,5-bisphosphate | wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
phosphate | wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
8.35 | - |
phosphate | mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. | |
9.02 | - |
phosphate | mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C | Synechocystis sp. |