Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.39 extracted from

  • Whitney, S.M.; Kane, H.J.; Houtz, R.L.; Sharwood, R.E.
    Rubisco oligomers composed of linked small and large subunits assemble in tobacco plastids and have higher affinities for CO2 and O2 (2009), Plant Physiol., 149, 1887-1895.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information the S and L subunits of Rubisco are linked together using a flexible 40-amino acid tether. By replacing the rbcL in tobacco plastids with an artificial gene coding for a S40L fusion peptide, the fusions readily assemble into catalytic (S40L)8 and (S40L)16 oligomers that are devoid of unlinked S subunits. While there is little or no change in CO2/O2 specificity or carboxylation rate of the Rubisco oligomers, their Kms for CO2 and O2 are reduced 10% to 20% and 45%, respectively. In young maturing leaves of the plastome transformants (ANtS40L), the S40L-Rubisco levels are approximately 20% that of wild-type controls despite turnover of the S40L-Rubisco oligomers being only slightly enhanced relative to wild-type Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
CO2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
0.0102
-
CO2 rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
0.0106
-
CO2 rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
0.0118
-
CO2 wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification Nicotiana tabacum
0.0122
-
CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
0.112
-
O2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
0.203
-
CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Q-sepharose anion exchange chromatography and gel filtration Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
Nicotiana tabacum 2 3-phospho-D-glycerate
-
?

Subunits

Subunits Comment Organism
hexadecamer wild-type enzyme is an assembly of eight plastid-encoded large (L) and eight nucleus-encoded small (S) subunits froming a hexadecameric (L8S8) enzyme Nicotiana tabacum

Synonyms

Synonyms Comment Organism
Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
Nicotiana tabacum
Rubisco
-
Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
O2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
0.65
-
CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
2.9
-
CO2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
2.9
-
CO2 rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
3.1
-
CO2 rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
3.1
-
CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
3.3
-
CO2 wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification Nicotiana tabacum