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Literature summary for 4.1.1.39 extracted from

  • Lilley, R.M.; Ralph, P.J.; Larkum, A.W.
    The Determination of Activity of the Enzyme Rubisco in Cell Extracts of the Dinoflagellate Alga Symbiodinium sp. by Manganese Chemiluminescence and its Response to Short-term Thermal Stress of the Alga (2010), Plant Cell Environ., 33, 995-1004.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a continuous assay for Rubisco activity in crude cell extracts using the Mn2+ chemiluminescence of Rubisco oxygenase is described Symbiodinium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Symbiodinium sp.

Organism

Organism UniProt Comment Textmining
Symbiodinium sp. Q41406
-
-

Purification (Commentary)

Purification (Comment) Organism
crude cell extracts bind [14C]2-carboxy-D-arabitinol 1,5-bisphosphate (CABP) to a high molecular mass fraction and CABP-Rubisco is separated by gel filtration chromatography Symbiodinium sp.

Storage Stability

Storage Stability Organism
-80°C, the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly Symbiodinium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Symbiodinium sp. ?
-
?

Synonyms

Synonyms Comment Organism
Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
Symbiodinium sp.
Rubisco
-
Symbiodinium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20.7
-
assay at Symbiodinium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-80
-
the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly Symbiodinium sp.
36
-
after pre-treatment of Symbiodinium cell cultures in darkness at temperatures above 30°C, the extracted Rubisco activities decrease, with almost complete loss of activity above 36°C Symbiodinium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Symbiodinium sp.