Application | Comment | Organism |
---|---|---|
molecular biology | a continuous assay for Rubisco activity in crude cell extracts using the Mn2+ chemiluminescence of Rubisco oxygenase is described | Symbiodinium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Symbiodinium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Symbiodinium sp. | Q41406 | - |
- |
Purification (Comment) | Organism |
---|---|
crude cell extracts bind [14C]2-carboxy-D-arabitinol 1,5-bisphosphate (CABP) to a high molecular mass fraction and CABP-Rubisco is separated by gel filtration chromatography | Symbiodinium sp. |
Storage Stability | Organism |
---|---|
-80°C, the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly | Symbiodinium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | - |
Symbiodinium sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Symbiodinium sp. |
Rubisco | - |
Symbiodinium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20.7 | - |
assay at | Symbiodinium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-80 | - |
the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly | Symbiodinium sp. |
36 | - |
after pre-treatment of Symbiodinium cell cultures in darkness at temperatures above 30°C, the extracted Rubisco activities decrease, with almost complete loss of activity above 36°C | Symbiodinium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Symbiodinium sp. |