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Literature summary for 4.1.1.39 extracted from

  • Carmo-Silva, A.E.; Keys, A.J.; Andralojc, P.J.; Powers, S.J.; Arrabaca, M.C.; Parry, M.A.
    Rubisco activities, properties, and regulation in three different C4 grasses under drought (2010), J. Exp. Bot., 61, 2355-2366.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-carboxyarabinitol-1-phosphate the inhibitor from darkened leaves of Zoysia japonica is identified as 2-carboxyarabinitol-1-phosphate. Consistent with the presence of 2-carboxyarabinitol-1-phosphate, the total activity of Rubisco is decreased after 12 h darkness in Zoysia japonica Zoysia japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00185
-
CO2 Vmax carboxylation: 3.78 micromol/min/mg Zoysia japonica
0.0021
-
CO2 Vmax carboxylation: 3.41 micromol/min/mg Cynodon dactylon
0.0109
-
CO2 Vmax carboxylation: 2.54 micromol/min/mg Paspalum dilatatum
0.019
-
CO2 Vmax carboxylation: 3.11 micromol/min/mg Paspalum dilatatum
0.341
-
O2 Vmax oxygenation: 0.79 micromol/min/mg Paspalum dilatatum
0.402
-
O2 Vmax oxygenation: 0.73 micromol/min/mg Cynodon dactylon
0.403
-
O2 Vmax oxygenation: 0.98 micromol/min/mg Zoysia japonica
0.415
-
O2 Vmax oxygenation: 0.71 micromol/min/mg Paspalum dilatatum

Organism

Organism UniProt Comment Textmining
Cynodon dactylon B0B766 fragment
-
Paspalum dilatatum B0B744 fragment
-
Zoysia japonica B0B758 fragment
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Paspalum dilatatum
-
leaf
-
Cynodon dactylon
-
leaf
-
Zoysia japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.73
-
light, control Cynodon dactylon
1.74
-
dark, control Zoysia japonica
1.74
-
dark, drought stress Zoysia japonica
1.79
-
light, drought stress Cynodon dactylon
1.81
-
dark, drought stress Cynodon dactylon
1.83
-
dark, control Cynodon dactylon
1.96
-
dark, drought stress Paspalum dilatatum
2.03
-
dark, control Paspalum dilatatum
2.11
-
light, control Paspalum dilatatum
2.14
-
light, drought stress Paspalum dilatatum
2.36
-
light, control Zoysia japonica
2.39
-
light, drought stress Zoysia japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Paspalum dilatatum ?
-
?
D-Ribulose 1,5-bisphosphate + CO2
-
Cynodon dactylon ?
-
?
D-Ribulose 1,5-bisphosphate + CO2
-
Zoysia japonica ?
-
?
D-ribulose 1,5-bisphosphate + O2
-
Paspalum dilatatum ?
-
?
D-ribulose 1,5-bisphosphate + O2
-
Cynodon dactylon ?
-
?
D-ribulose 1,5-bisphosphate + O2
-
Zoysia japonica ?
-
?

Synonyms

Synonyms Comment Organism
Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
Paspalum dilatatum
Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
Cynodon dactylon
Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
Zoysia japonica
Rubisco
-
Paspalum dilatatum
Rubisco
-
Cynodon dactylon
Rubisco
-
Zoysia japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Paspalum dilatatum
25
-
assay at Cynodon dactylon
25
-
assay at Zoysia japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
assay at Paspalum dilatatum
8.2
-
assay at Cynodon dactylon
8.2
-
assay at Zoysia japonica

General Information

General Information Comment Organism
physiological function Rubisco initial and total activities decrease slightly with water deficit, but maximal activity and Rubisco quantity remain unchanged, consistent with commonly observed drought effects. No significant differences in Rubisco total activities are observed between the light and dark-adapted leaves of Paspalum dilatatum and Cynodon dactylon and therefore no evidence is shown of a bound inhibitor for these two species. In neither light nor dark is there evidence of an increase in tight-binding inhibitor with drought stress Paspalum dilatatum
physiological function Rubisco initial and total activities decrease slightly with water deficit, but maximal activity and Rubisco quantity remain unchanged, consistent with commonly observed drought effects. No significant differences in Rubisco total activities are observed between the light and dark-adapted leaves of Paspalum dilatatum and Cynodon dactylon and therefore no evidence is shown of a bound inhibitor for these two species. In neither light nor dark is there evidence of an increase in tight-binding inhibitor with drought stress Cynodon dactylon
physiological function Rubisco initial and total activities decrease slightly with water deficit, but maximal activity and Rubisco quantity remain unchanged, consistent with commonly observed drought effects. Significant differences in Rubisco total activities are observed between the light and dark-adapted leaves of Zoysia japonica. The total activities of Rubisco in dark-adapted leaves of this species (after the 12 h dark period) are 74% of the total activities in leaves harvested in the light, meaning that inhibitors are bound to 26% of the catalytic sites in the dark. This result is independent of water deficit Zoysia japonica