Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein and in tobacco chloroplasts | Methanococcoides burtonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-carboxy-D-arabinitol 1,5-bisphosphate | - |
Methanococcoides burtonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
D-ribulose 1,5-bisphosphate | pentamer | Methanococcoides burtonii | |
0.13 | - |
CO2 | pentamer | Methanococcoides burtonii | |
2.5 | - |
O2 | pentamer | Methanococcoides burtonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits activity and promotes pentamer formation | Methanococcoides burtonii | |
Co2+ | inhibits activity and promotes pentamer formation | Methanococcoides burtonii | |
Mg2+ | - |
Methanococcoides burtonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52857 | - |
2 * 52857, nondenaturing PAGE analysis, MbR assembles into functional dimers when expressed in Escherichia coli. The catalytic properties of the dimer and the pentamer of MbR are indistinguishable | Methanococcoides burtonii |
52860 | - |
monomer, nano ESI-MS | Methanococcoides burtonii |
105700 | - |
dimer, nano ESI-MS | Methanococcoides burtonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcoides burtonii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using metal affinity chromatography | Methanococcoides burtonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | - |
Methanococcoides burtonii | ? | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Methanococcoides burtonii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52857, nondenaturing PAGE analysis, MbR assembles into functional dimers when expressed in Escherichia coli. The catalytic properties of the dimer and the pentamer of MbR are indistinguishable | Methanococcoides burtonii |
pentamer | assembly into pentamers of L2 (L10) occurs when expressed in tobacco chloroplasts or Escherichia coli producing RuBP. In vitro analyses indicate that the sequential assembly of L2 into L10 occurs without chaperone involvement and is stimulated by protein rearrangements associated with either the binding of substrate RuBP, the tight binding transition state analog carboxyarabinitol-1,5-bisphosphate, or inhibitory divalent metal ions within the active site | Methanococcoides burtonii |
Synonyms | Comment | Organism |
---|---|---|
MbR | - |
Methanococcoides burtonii |
Rubisco | - |
Methanococcoides burtonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, pretreatment at 45°C for 10 min | Methanococcoides burtonii |
55 | - |
for both dimer and pentamer | Methanococcoides burtonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
D-ribulose 1,5-bisphosphate | pH 8.0, for both dimer and pentamer | Methanococcoides burtonii | |
4 | - |
D-ribulose 1,5-bisphosphate | pH 6.0, for both dimer and pentamer | Methanococcoides burtonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanococcoides burtonii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
2-carboxy-D-arabinitol 1,5-bisphosphate | - |
Methanococcoides burtonii |