Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Chlamydomonas reinhardtii |
Crystallization (Comment) | Organism |
---|---|
analysis of the 1.4-A X-ray crystal structure of Chlamydomonas Rubisco reveal that all but one (Glu-92) of the conserved residues are in contact with large subunits and cluster near the amino- or carboxyl-terminal ends of large subunit alpha-helix 8, which is a structural element of the alpha/beta-barrel active site. Small subunit residues Glu-43 and Trp-73 identify a possible structural connection between active site alpha-helix 8 and the highly variable small subunit loop between beta-strands A and B, which can also influence Rubisco CO2/O2 specificity | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
E43A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant causes decreases in CO2/O2 specificity, Km (CO2) increased compared to wild-type, Km (O2) highly increased compared to wild-type | Chlamydomonas reinhardtii |
E92A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant shows a phenotype comparable to wild-type | Chlamydomonas reinhardtii |
F81A | mutant restores photosynthetic growth to the rbcS knock-out strain, Km (CO2) increased compared to wild-type, Km (O2) highly increased compared to wild-type | Chlamydomonas reinhardtii |
L18A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant affects holoenzyme stability | Chlamydomonas reinhardtii |
L78A | mutant restores photosynthetic growth to the rbcS knock-out strain, Km (CO2) increased compared to wild-type, Km (O2) highly increased compared to wild-type | Chlamydomonas reinhardtii |
P19A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant shows a phenotype comparable to wild-type | Chlamydomonas reinhardtii |
P79A | mutant restores photosynthetic growth to the rbcS knock-out strain, Km (CO2) equal to wild-type, Km (O2) increased compared to wild-type | Chlamydomonas reinhardtii |
S16A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant shows a phenotype comparable to wild-type | Chlamydomonas reinhardtii |
W73A | mutant restores photosynthetic growth to the rbcS knock-out strain, Km (CO2) increased compared to wild-type, Km (O2) highly increased compared to wild-type | Chlamydomonas reinhardtii |
Y32A | mutant restores photosynthetic growth to the rbcS knock-out strain, mutant causes decreases in CO2/O2 specificity, Km (CO2) equal to wild-type, Km (O2) decreased compared to wild-type | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
CO2 | mutant Y32A | Chlamydomonas reinhardtii | |
0.031 | - |
CO2 | wild-type | Chlamydomonas reinhardtii | |
0.033 | - |
CO2 | mutant P79A | Chlamydomonas reinhardtii | |
0.043 | - |
CO2 | mutant F81A | Chlamydomonas reinhardtii | |
0.046 | - |
CO2 | mutant W73A | Chlamydomonas reinhardtii | |
0.053 | - |
CO2 | mutant L78A | Chlamydomonas reinhardtii | |
0.064 | - |
CO2 | mutant E43A | Chlamydomonas reinhardtii | |
0.383 | - |
O2 | mutant Y32A | Chlamydomonas reinhardtii | |
0.527 | - |
O2 | wild-type | Chlamydomonas reinhardtii | |
0.529 | - |
O2 | mutant P79A | Chlamydomonas reinhardtii | |
0.974 | - |
O2 | mutant L78A | Chlamydomonas reinhardtii | |
1.008 | - |
O2 | mutant F81A | Chlamydomonas reinhardtii | |
1.159 | - |
O2 | mutant W73A | Chlamydomonas reinhardtii | |
1.624 | - |
O2 | mutant E43A | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | - |
Chlamydomonas reinhardtii | ? | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Chlamydomonas reinhardtii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rbcS1 | - |
Chlamydomonas reinhardtii |
Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Chlamydomonas reinhardtii |
Rubisco | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chlamydomonas reinhardtii |