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Literature summary for 4.1.1.39 extracted from

  • Liggins, J.R.; Gready, J.E.
    Putative functional role for the invariant aspartate 263 residue of Rhodospirillum rubrum Rubisco (2009), Biochemistry, 48, 2226-2236.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
D263A kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype Rhodospirillum rubrum
D263E kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263E has a significantly higher Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) Rhodospirillum rubrum
D263N kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263N has a significantly lower Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) Rhodospirillum rubrum
D263Q D263Q shows the lowest value for kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) Rhodospirillum rubrum
D263S kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
D-ribulose 1,5-bisphosphate mutant D263Q Rhodospirillum rubrum
0.0042
-
D-ribulose 1,5-bisphosphate mutant D263E Rhodospirillum rubrum
0.005
-
D-ribulose 1,5-bisphosphate mutant D263A Rhodospirillum rubrum
0.0089
-
D-ribulose 1,5-bisphosphate mutant D263S Rhodospirillum rubrum
0.009
-
CO2 mutant D263S Rhodospirillum rubrum
0.0098
-
D-ribulose 1,5-bisphosphate mutant D263N Rhodospirillum rubrum
0.0128
-
D-ribulose 1,5-bisphosphate wild-type Rhodospirillum rubrum
0.014
-
CO2 mutant D263Q Rhodospirillum rubrum
0.018
-
CO2 mutant D263A Rhodospirillum rubrum
0.026
-
CO2 mutant D263N Rhodospirillum rubrum
0.076
-
CO2 mutant D263E Rhodospirillum rubrum
0.446
-
CO2 wild-type Rhodospirillum rubrum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum P04718
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Rhodospirillum rubrum ?
-
?

Synonyms

Synonyms Comment Organism
Ribulose 1,5-bisphosphate carboxylase
-
Rhodospirillum rubrum
Rubisco
-
Rhodospirillum rubrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rhodospirillum rubrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
D-ribulose 1,5-bisphosphate mutant D263Q, cosubstrate: CO2 Rhodospirillum rubrum
0.2
-
CO2 mutant D263Q, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
0.46
-
D-ribulose 1,5-bisphosphate mutant D263S, cosubstrate: CO2 Rhodospirillum rubrum
0.46
-
CO2 mutant D263S, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
0.81
-
D-ribulose 1,5-bisphosphate mutant D263A, cosubstrate: CO2 Rhodospirillum rubrum
0.81
-
CO2 mutant D263A, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
0.82
-
D-ribulose 1,5-bisphosphate mutant D263N, cosubstrate: CO2 Rhodospirillum rubrum
0.82
-
CO2 mutant D263N, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
0.94
-
D-ribulose 1,5-bisphosphate mutant D263E, cosubstrate: CO2 Rhodospirillum rubrum
0.94
-
CO2 mutant D263E, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum
6.28
-
D-ribulose 1,5-bisphosphate wild-type, cosubstrate: CO2 Rhodospirillum rubrum
6.28
-
CO2 wild-type, cosubstrate: D-ribulose 1,5-bisphosphate Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rhodospirillum rubrum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
CO2 mutant D263E Rhodospirillum rubrum
14
-
CO2 wild-type Rhodospirillum rubrum
15
-
CO2 mutant D263Q Rhodospirillum rubrum
32
-
CO2 mutant D263N Rhodospirillum rubrum
44
-
CO2 mutant D263A Rhodospirillum rubrum
49
-
CO2 mutant D263S Rhodospirillum rubrum
52
-
D-ribulose 1,5-bisphosphate mutant D263S Rhodospirillum rubrum
84
-
D-ribulose 1,5-bisphosphate mutant D263N Rhodospirillum rubrum
160
-
D-ribulose 1,5-bisphosphate mutant D263A Rhodospirillum rubrum
160
-
D-ribulose 1,5-bisphosphate mutant D263Q Rhodospirillum rubrum
220
-
D-ribulose 1,5-bisphosphate mutant D263E Rhodospirillum rubrum
490
-
D-ribulose 1,5-bisphosphate wild-type Rhodospirillum rubrum