Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
D263A | kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype | Rhodospirillum rubrum |
D263E | kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263E has a significantly higher Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) | Rhodospirillum rubrum |
D263N | kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype, mutant D263N has a significantly lower Km value for (substrate: CO2) compared to mutant D263N (although both mutants have approximately the same kcat value) | Rhodospirillum rubrum |
D263Q | D263Q shows the lowest value for kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) | Rhodospirillum rubrum |
D263S | kcat and Km (substrate: CO2) (substrate: D-ribulose 1,5-bisphosphate) are reduced compared to wildtype | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
D-ribulose 1,5-bisphosphate | mutant D263Q | Rhodospirillum rubrum | |
0.0042 | - |
D-ribulose 1,5-bisphosphate | mutant D263E | Rhodospirillum rubrum | |
0.005 | - |
D-ribulose 1,5-bisphosphate | mutant D263A | Rhodospirillum rubrum | |
0.0089 | - |
D-ribulose 1,5-bisphosphate | mutant D263S | Rhodospirillum rubrum | |
0.009 | - |
CO2 | mutant D263S | Rhodospirillum rubrum | |
0.0098 | - |
D-ribulose 1,5-bisphosphate | mutant D263N | Rhodospirillum rubrum | |
0.0128 | - |
D-ribulose 1,5-bisphosphate | wild-type | Rhodospirillum rubrum | |
0.014 | - |
CO2 | mutant D263Q | Rhodospirillum rubrum | |
0.018 | - |
CO2 | mutant D263A | Rhodospirillum rubrum | |
0.026 | - |
CO2 | mutant D263N | Rhodospirillum rubrum | |
0.076 | - |
CO2 | mutant D263E | Rhodospirillum rubrum | |
0.446 | - |
CO2 | wild-type | Rhodospirillum rubrum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | P04718 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | - |
Rhodospirillum rubrum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ribulose 1,5-bisphosphate carboxylase | - |
Rhodospirillum rubrum |
Rubisco | - |
Rhodospirillum rubrum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rhodospirillum rubrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-ribulose 1,5-bisphosphate | mutant D263Q, cosubstrate: CO2 | Rhodospirillum rubrum | |
0.2 | - |
CO2 | mutant D263Q, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum | |
0.46 | - |
D-ribulose 1,5-bisphosphate | mutant D263S, cosubstrate: CO2 | Rhodospirillum rubrum | |
0.46 | - |
CO2 | mutant D263S, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum | |
0.81 | - |
D-ribulose 1,5-bisphosphate | mutant D263A, cosubstrate: CO2 | Rhodospirillum rubrum | |
0.81 | - |
CO2 | mutant D263A, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum | |
0.82 | - |
D-ribulose 1,5-bisphosphate | mutant D263N, cosubstrate: CO2 | Rhodospirillum rubrum | |
0.82 | - |
CO2 | mutant D263N, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum | |
0.94 | - |
D-ribulose 1,5-bisphosphate | mutant D263E, cosubstrate: CO2 | Rhodospirillum rubrum | |
0.94 | - |
CO2 | mutant D263E, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum | |
6.28 | - |
D-ribulose 1,5-bisphosphate | wild-type, cosubstrate: CO2 | Rhodospirillum rubrum | |
6.28 | - |
CO2 | wild-type, cosubstrate: D-ribulose 1,5-bisphosphate | Rhodospirillum rubrum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodospirillum rubrum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
CO2 | mutant D263E | Rhodospirillum rubrum | |
14 | - |
CO2 | wild-type | Rhodospirillum rubrum | |
15 | - |
CO2 | mutant D263Q | Rhodospirillum rubrum | |
32 | - |
CO2 | mutant D263N | Rhodospirillum rubrum | |
44 | - |
CO2 | mutant D263A | Rhodospirillum rubrum | |
49 | - |
CO2 | mutant D263S | Rhodospirillum rubrum | |
52 | - |
D-ribulose 1,5-bisphosphate | mutant D263S | Rhodospirillum rubrum | |
84 | - |
D-ribulose 1,5-bisphosphate | mutant D263N | Rhodospirillum rubrum | |
160 | - |
D-ribulose 1,5-bisphosphate | mutant D263A | Rhodospirillum rubrum | |
160 | - |
D-ribulose 1,5-bisphosphate | mutant D263Q | Rhodospirillum rubrum | |
220 | - |
D-ribulose 1,5-bisphosphate | mutant D263E | Rhodospirillum rubrum | |
490 | - |
D-ribulose 1,5-bisphosphate | wild-type | Rhodospirillum rubrum |