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Literature summary for 4.1.1.39 extracted from

  • Gubernator, B.; Bartoszewski, R.; Kroliczewski, J.; Wildner, G.; Szczepaniak, A.
    Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus (2008), Photosynth. Res., 95, 101-109.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pUC18 plasmid for expression in Escherichia coli DH5alpha cells Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0204
-
D-ribulose 1,5-bisphosphate
-
Thermosynechococcus vestitus
0.0543
-
CO2
-
Thermosynechococcus vestitus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
small subunit, determined by SDS-PAGE Thermosynechococcus vestitus
53000
-
large subunit, determined by SDS-PAGE Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glycerate + H+ Thermosynechococcus vestitus
-
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrapQ DEAE sepharose and a MonoQ column Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
purification step crude extract Thermosynechococcus vestitus
0.5
-
purification step DEAE sepharose eluate Thermosynechococcus vestitus
1.83
-
purification step MonoQ eluate Thermosynechococcus vestitus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + H+
-
Thermosynechococcus vestitus D-ribulose 1,5-bisphosphate + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
multimer
-
Thermosynechococcus vestitus

Synonyms

Synonyms Comment Organism
green-like type rubisco
-
Thermosynechococcus vestitus
ribulose-1,5-bisphosphate carboxylase/oxygenase
-
Thermosynechococcus vestitus
Rubisco
-
Thermosynechococcus vestitus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
rubisco carboxylase assay Thermosynechococcus vestitus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the enzyme is stable and active up to 70°C and deactivated at 75°C Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.65
-
CO2
-
Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
rubisco carboxylase assay Thermosynechococcus vestitus