Cloned (Comment) | Organism |
---|---|
into the pUC18 plasmid for expression in Escherichia coli DH5alpha cells | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0204 | - |
D-ribulose 1,5-bisphosphate | - |
Thermosynechococcus vestitus | |
0.0543 | - |
CO2 | - |
Thermosynechococcus vestitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
small subunit, determined by SDS-PAGE | Thermosynechococcus vestitus |
53000 | - |
large subunit, determined by SDS-PAGE | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + H+ | Thermosynechococcus vestitus | - |
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HiTrapQ DEAE sepharose and a MonoQ column | Thermosynechococcus vestitus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
purification step crude extract | Thermosynechococcus vestitus |
0.5 | - |
purification step DEAE sepharose eluate | Thermosynechococcus vestitus |
1.83 | - |
purification step MonoQ eluate | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + H+ | - |
Thermosynechococcus vestitus | D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | - |
Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
green-like type rubisco | - |
Thermosynechococcus vestitus |
ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Thermosynechococcus vestitus |
Rubisco | - |
Thermosynechococcus vestitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
rubisco carboxylase assay | Thermosynechococcus vestitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the enzyme is stable and active up to 70°C and deactivated at 75°C | Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.65 | - |
CO2 | - |
Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
rubisco carboxylase assay | Thermosynechococcus vestitus |