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Literature summary for 4.1.1.39 extracted from

  • Kreel, N.E.; Tabita, F.R.
    Substitutions at methionine 295 of Archaeoglobus fulgidus ribulose-1,5-bisphosphate carboxylase/oxygenase affect oxygen binding and CO2/O2 specificity (2007), J. Biol. Chem., 282, 1341-1351.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
M295D less sensitive to oxygen compared with the wild-type enzyme, retains 35-40% of activity after exposing to O2 Archaeoglobus fulgidus
M295D/S363I less sensitive to oxygen compared with the wild-type enzyme, retains 82% of activity after exposing to O2 Archaeoglobus fulgidus
M295D/S363V less sensitive to oxygen compared with the wild-type enzyme, retains 86% of activity after exposing to O2 Archaeoglobus fulgidus
S363I less sensitive to oxygen compared with the wild-type enzyme Archaeoglobus fulgidus
S363V less sensitive to oxygen compared with the wild-type enzyme Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
O2 competitive inhibitor with respect to CO2, assay in the presence of oxygen and high levels of CO2 results in substantial loss (85-90%) of activity compared with assays performed under strictly anaerobic conditions Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
O2 wild type enzyme, at 83°C Archaeoglobus fulgidus
0.0205
-
D-ribulose 1,5-bisphosphate wild type enzyme, at 83°C Archaeoglobus fulgidus
0.021
-
D-ribulose 1,5-bisphosphate mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
0.024
-
O2 mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
0.051
-
CO2 wild type enzyme, at 83°C Archaeoglobus fulgidus
0.058
-
CO2 mutant enzyme M295D, at 83°C Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48500
-
2 * 48500, SDS-PAGE Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superose 12 gel filtration, and phenyl-Sepharose column chromatography Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
Archaeoglobus fulgidus 3-phospho-D-glycerate
-
?
D-ribulose 1,5-bisphosphate + O2
-
Archaeoglobus fulgidus 3-phospho-D-glycerate + 2-phosphoglycolate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 48500, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
form III ribulose-1,5-bisphosphate carboxylase/oxygenase
-
Archaeoglobus fulgidus
RbcL2
-
Archaeoglobus fulgidus
Rubisco
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
83
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 93
-
Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.7
-
D-ribulose 1,5-bisphosphate mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
23.1
-
D-ribulose 1,5-bisphosphate wild type enzyme, at 83°C Archaeoglobus fulgidus