Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
M295D | less sensitive to oxygen compared with the wild-type enzyme, retains 35-40% of activity after exposing to O2 | Archaeoglobus fulgidus |
M295D/S363I | less sensitive to oxygen compared with the wild-type enzyme, retains 82% of activity after exposing to O2 | Archaeoglobus fulgidus |
M295D/S363V | less sensitive to oxygen compared with the wild-type enzyme, retains 86% of activity after exposing to O2 | Archaeoglobus fulgidus |
S363I | less sensitive to oxygen compared with the wild-type enzyme | Archaeoglobus fulgidus |
S363V | less sensitive to oxygen compared with the wild-type enzyme | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | competitive inhibitor with respect to CO2, assay in the presence of oxygen and high levels of CO2 results in substantial loss (85-90%) of activity compared with assays performed under strictly anaerobic conditions | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
O2 | wild type enzyme, at 83°C | Archaeoglobus fulgidus | |
0.0205 | - |
D-ribulose 1,5-bisphosphate | wild type enzyme, at 83°C | Archaeoglobus fulgidus | |
0.021 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme M295D, at 83°C | Archaeoglobus fulgidus | |
0.024 | - |
O2 | mutant enzyme M295D, at 83°C | Archaeoglobus fulgidus | |
0.051 | - |
CO2 | wild type enzyme, at 83°C | Archaeoglobus fulgidus | |
0.058 | - |
CO2 | mutant enzyme M295D, at 83°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48500 | - |
2 * 48500, SDS-PAGE | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superose 12 gel filtration, and phenyl-Sepharose column chromatography | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Archaeoglobus fulgidus | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Archaeoglobus fulgidus | 3-phospho-D-glycerate + 2-phosphoglycolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48500, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
form III ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Archaeoglobus fulgidus |
RbcL2 | - |
Archaeoglobus fulgidus |
Rubisco | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 93 | - |
Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.7 | - |
D-ribulose 1,5-bisphosphate | mutant enzyme M295D, at 83°C | Archaeoglobus fulgidus | |
23.1 | - |
D-ribulose 1,5-bisphosphate | wild type enzyme, at 83°C | Archaeoglobus fulgidus |