Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue cells | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
D117H | 40% reduced CO2/O2 specificity | Rhodospirillum rubrum |
D117V | 40% reduced CO2/O2 specificity | Rhodospirillum rubrum |
H44N | 40% reduced CO2/O2 specificity | Rhodospirillum rubrum |
H44Q | 40% reduced CO2/O2 specificity | Rhodospirillum rubrum |
K191M | inactive | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1,6-bisphosphate | - |
Rhodospirillum rubrum | |
O2 | - |
Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.149 | - |
CO2 | wild type enzyme, at 25 °C, pH 8 | Rhodospirillum rubrum | |
0.192 | - |
CO2 | mutant enzyme D117H, at 25 °C, pH 8 | Rhodospirillum rubrum | |
0.196 | - |
CO2 | mutant enzyme D117V, at 25 °C, pH 8 | Rhodospirillum rubrum | |
0.204 | - |
CO2 | mutant enzyme H44N, at 25 °C, pH 8 | Rhodospirillum rubrum | |
0.301 | - |
CO2 | mutant enzyme H44Q, at 25 °C, pH 8 | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | P04718 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Rhodospirillum rubrum | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Rhodospirillum rubrum | 3-phospho-D-glycerate + 2-phosphoglycolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rhodospirillum rubrum |
Synonyms | Comment | Organism |
---|---|---|
Form II Rubisco | dimeric form | Rhodospirillum rubrum |
ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Rhodospirillum rubrum |
Rubisco | - |
Rhodospirillum rubrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
O2 | mutant enzyme H44N, at 25°C, pH 8 | Rhodospirillum rubrum | |
1.1 | - |
O2 | mutant enzyme D117H, at 25°C, pH 8 | Rhodospirillum rubrum | |
1.2 | - |
O2 | mutant enzyme H44Q, at 25°C, pH 8 | Rhodospirillum rubrum | |
1.4 | - |
O2 | wild type enzyme, at 25°C, pH 8 | Rhodospirillum rubrum | |
1.5 | - |
O2 | mutant enzyme D117V, at 25°C, pH 8 | Rhodospirillum rubrum | |
7.4 | - |
CO2 | mutant enzyme D117V, at 25°C, pH 8 | Rhodospirillum rubrum | |
7.5 | - |
CO2 | mutant enzyme D117H, at 25°C, pH 8 | Rhodospirillum rubrum | |
9.3 | - |
CO2 | mutant enzyme H44Q, at 25°C, pH 8 | Rhodospirillum rubrum | |
9.8 | - |
CO2 | mutant enzyme H44N, at 25°C, pH 8 | Rhodospirillum rubrum | |
12.3 | - |
CO2 | wild type enzyme, at 25°C, pH 8 | Rhodospirillum rubrum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
O2 | mutant enzyme H44N, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.153 | - |
O2 | mutant enzyme D117H, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.159 | - |
O2 | wild type enzyme, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.185 | - |
O2 | mutant enzyme H44Q, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.199 | - |
O2 | mutant enzyme D117V, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.245 | - |
D-fructose-1,6-bisphosphate | mutant enzyme D117H, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.32 | - |
D-fructose-1,6-bisphosphate | mutant enzyme H44N, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.347 | - |
D-fructose-1,6-bisphosphate | mutant enzyme D117V, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.348 | - |
D-fructose-1,6-bisphosphate | mutant enzyme H44Q, at 25°C, pH 8 | Rhodospirillum rubrum | |
0.465 | - |
D-fructose-1,6-bisphosphate | wild type enzyme, at 25°C, pH 8 | Rhodospirillum rubrum |