Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 50 mM HEPES (pH 7.5), 0.05-0.2 M NaCl, 7-12% poly(ethylene glycol) 4000, 10 mM NaHCO3, and 5 mM MgCl2 | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
D473E | altered CO2/O2 specificity | Chlamydomonas reinhardtii |
G344S | decrease in CO2/O2 specificity | Chlamydomonas reinhardtii |
T342I | altered CO2/O2 specificity | Chlamydomonas reinhardtii |
T342I | decrease in CO2/O2 specificity | Chlamydomonas reinhardtii |
V331A | altered CO2/O2 specificity | Chlamydomonas reinhardtii |
V331A/G344S | altered CO2/O2 specificity | Chlamydomonas reinhardtii |
V331A/T342I | altered CO2/O2 specificity | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Chlamydomonas reinhardtii 2137 mt+ | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superdex-200 Hiload 16/60 gel filtration and MonoQ anion exchange column chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Chlamydomonas reinhardtii | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Chlamydomonas reinhardtii 2137 mt+ | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Chlamydomonas reinhardtii | 3-phospho-D-glycerate + 2-phosphoglycolate | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Chlamydomonas reinhardtii 2137 mt+ | 3-phospho-D-glycerate + 2-phosphoglycolate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Chlamydomonas reinhardtii |
Rubisco | - |
Chlamydomonas reinhardtii |