Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | a solution containing 10 mM NaHCO3, 10 mM MgCl2, and 10 mM Bicine-NaOH (pH 8.3) is used for enzyme activation | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Mutants SP1 and SP2 harbor mutations in the nonconserved residues of loop 6 leading to strongly decreased specific activities. Within alpha-helix 6, SP3 contains a single amino acid substitution from valine to threonine leading to increased specific activity. Mutant SP4 has substitutions in five consecutive amino acid residues leading to increased specific activity. SP5 has a 6-amino-acid substitution in alpha-helix 6 leading to decreased specific activity, while SP6 has 11 substitutions in alpha-helix 6 leading to 31% increased specific activity. In SP7, the entire loop 6/alpha-helix 6 region is replaced with the corresponding region of spinach Rubisco resulting in strongly depressed specific activity. | Thermococcus kodakarensis |
V330T | negative effect on Rbc activity | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
CO2 | wild type enzyme, at 25°C | Thermococcus kodakarensis | |
0.9 | - |
O2 | wild type enzyme, at 25°C | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PD-10 gel filtration | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Thermococcus kodakarensis | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + O2 | - |
Thermococcus kodakarensis | 3-phospho-D-glycerate + 2-phosphoglycolate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rbc | - |
Thermococcus kodakarensis |
Rubisco | - |
Thermococcus kodakarensis |
type III ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 100 | the half life of the wild type enzyme is about 5fold lower at 100°C than at 90°C | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
CO2 | wild type enzyme, at 25°C | Thermococcus kodakarensis | |
0.38 | - |
O2 | wild type enzyme, at 25°C | Thermococcus kodakarensis |