Literature summary for 4.1.1.39 extracted from

Spreitzer, R.J.; Peddi, S.R.; Satagopan, S.
Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco (2005), Proc. Natl. Acad. Sci. USA, 102, 17225-17230.

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Protein Variants

Protein Variants	Comment	Organism
C256F/K258R/I265V	mutant strain grows poorly on minimal medium at 35°C	Chlamydomonas reinhardtii
V221C/V235I	growth phenotype of mutant strain is indistinguishable from wild-type, kinetic constants are not significantly different from those of wild-type enzyme	Chlamydomonas reinhardtii
V221C/V235I/C256F/K258R/I265V	mutant grows significantly better than mutant C256F/K258R/I265V on minimal medium at 35°C	Chlamydomonas reinhardtii
V235I	growth phenotype of mutant strain is indistinguishable from wild-type, kinetic constants are not significantly different from those of wild-type enzyme	Chlamydomonas reinhardtii

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-

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Release 2023.1 (January 2023)