Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
D69S | site-directed mutagenesis, decameric between 30°C-90°C | Thermococcus kodakarensis |
E63S | site-directed mutagenesis, decamer at 30°C, gradually dissebles with elevation in temperature | Thermococcus kodakarensis |
E63S/R66S/D69S | site-directed mutagenesis, is found in a dimeric form even at 30°C | Thermococcus kodakarensis |
R66S | site-directed mutagenesis, decamer at 30°C, gradually dissebles with elevation in temperature | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | Thermococcus kodakarensis | key enzyme in the Calvin-Benson cycle, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant enzyme on our planet | 3-phospho-D-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | O93627 | KOD1 | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant proteins | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Thermococcus kodakarensis | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + CO2 + H2O | key enzyme in the Calvin-Benson cycle, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant enzyme on our planet | Thermococcus kodakarensis | 3-phospho-D-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | wild-type and mutant D69S | Thermococcus kodakarensis |
dimer | mutant E63S/R66S/D69S | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
archaeal Rubisco | - |
Thermococcus kodakarensis |
Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Thermococcus kodakarensis |
Rubisco | - |
Thermococcus kodakarensis |
Tk-Rubisco | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | - |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 100 | stable against this treatment for 30 min, denaturation above 113°C | Thermococcus kodakarensis |