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Literature summary for 4.1.1.39 extracted from

  • Simpson, S.A.; Lawlis, V.B.; Mueller, D.D.
    Ribulose-1,5-bisphosphate carboxylase from comfrey: isolation and characterization (1983), Phytochemistry, 22, 1121-1125.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
D-ribulose 1,5-bisphosphate
-
Symphytum sp.
0.13
-
CO2
-
Symphytum sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Symphytum sp. 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Symphytum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12700
-
8 * 50000 + 8 * 12700, SDS-PAGE Symphytum sp.
50000
-
8 * 50000 + 8 * 12700, SDS-PAGE Symphytum sp.
501600
-
calcuation from stoichiometry of subunits Symphytum sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2 Symphytum sp.
-
?
-
?

Organism

Organism UniProt Comment Textmining
Symphytum sp.
-
comfrey
-

Purification (Commentary)

Purification (Comment) Organism
-
Symphytum sp.

Reaction

Reaction Comment Organism Reaction ID
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Symphytum sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Symphytum sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
carboxylase Symphytum sp.

Storage Stability

Storage Stability Organism
frozen, 0.53 M NaCl Symphytum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Symphytum sp. D-3-Phosphoglycerate
-
?
D-Ribulose 1,5-bisphosphate + CO2
-
Symphytum sp. ?
-
?

Subunits

Subunits Comment Organism
hexadecamer 8 * 50000 + 8 * 12700, SDS-PAGE Symphytum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Symphytum sp.