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Literature summary for 4.1.1.39 extracted from

  • Servaites, J.C.
    Crystalline ribulose bisphosphate carboxylase/oxygenase of high integrity and catalytic activity from Nicotiana tabacum (1985), Arch. Biochem. Biophys., 238, 154-160.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Nicotiana tabacum

General Stability

General Stability Organism
casein and leupeptin protect against proteolysis Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009 0.013 D-ribulose 1,5-bisphosphate
-
Nicotiana tabacum
0.012 0.015 CO2
-
Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Nicotiana tabacum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
x * 55000 + x * 14000, SDS-PAGE Nicotiana tabacum
55000
-
x * 55000 + x * 14000, SDS-PAGE Nicotiana tabacum
515000
-
gel filtration Nicotiana tabacum
538000
-
gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2 Nicotiana tabacum
-
?
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Reaction

Reaction Comment Organism Reaction ID
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana tabacum
-

Storage Stability

Storage Stability Organism
4°C, 25 mM Tris-HCl, pH 7.2, 0.1 mM EDTA, 2 months stable Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Nicotiana tabacum D-3-Phosphoglycerate
-
?
D-Ribulose 1,5-bisphosphate + CO2
-
Nicotiana tabacum ?
-
?

Subunits

Subunits Comment Organism
? x * 55000 + x * 14000, SDS-PAGE Nicotiana tabacum