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Literature summary for 4.1.1.39 extracted from

  • Holuigue, L.; Herrera, L.; Phillips, O.M.; Young, M.; Alende, J.E.
    CO2 fixation by mineral-leaching bacteria: characteristics of the ribulose bisphosphate carboxylase-oxygenase of Thiobacilllus ferrooxidans (1987), Biotechnol. Appl. Biochem., 9, 497-505.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate
-
Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
CO2
-
Acidithiobacillus ferrooxidans
0.08
-
D-ribulose 1,5-bisphosphate
-
Acidithiobacillus ferrooxidans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Acidithiobacillus ferrooxidans 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans
54000
-
A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans
570000
-
non-denaturing gel electrophoresis Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2 Acidithiobacillus ferrooxidans
-
?
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Acidithiobacillus ferrooxidans

Reaction

Reaction Comment Organism Reaction ID
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
112
-
carboxylase Acidithiobacillus ferrooxidans

Storage Stability

Storage Stability Organism
4°C, 70% (NH4)2SO4 Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 1,5-bisphosphate + CO2
-
Acidithiobacillus ferrooxidans D-3-Phosphoglycerate
-
?
D-Ribulose 1,5-bisphosphate + CO2
-
Acidithiobacillus ferrooxidans ?
-
?

Subunits

Subunits Comment Organism
hexadecamer A8B8, 8 * 54000 (A) + 8 * 15500 (B), SDS-PAGE Acidithiobacillus ferrooxidans