Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ricinus communis |
Protein Variants | Comment | Organism |
---|---|---|
S425A | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
S425A/S451D | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
S451D | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
phosphoenolpyruvate | wild type enzyme, at pH 7.3 and 25°C | Ricinus communis | |
0.76 | - |
phosphoenolpyruvate | wild type enzyme, at pH 8.0 and 25°C | Ricinus communis | |
1 | - |
phosphoenolpyruvate | mutant enzyme S425A/S451D, at pH 7.3 and 25°C | Ricinus communis | |
1.6 | - |
phosphoenolpyruvate | mutant enzyme S425A/S451D, at pH 8.0 and 25°C | Ricinus communis | |
2 | - |
phosphoenolpyruvate | mutant enzyme S425A, at pH 7.3 and 25°C | Ricinus communis | |
2.1 | - |
phosphoenolpyruvate | mutant enzyme S451D, at pH 7.3 and 25°C | Ricinus communis | |
2.4 | - |
phosphoenolpyruvate | mutant enzyme S451D, at pH 8.0 and 25°C | Ricinus communis | |
2.7 | - |
phosphoenolpyruvate | mutant enzyme S425A, at pH 8.0 and 25°C | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
900000 | - |
gel filtration | Ricinus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | Ricinus communis | - |
phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
cultivar Baker 296 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is in vivo phosphorylated at Ser451 | Ricinus communis |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity resin column chromatography, Superdex 200 gel filtration, and Superose-6 gel filtration | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Ricinus communis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Ricinus communis | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterooctamer | - |
Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
PEPC | - |
Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Ricinus communis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Ricinus communis |