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Literature summary for 4.1.1.31 extracted from

  • Chen, Z.; Bommareddy, R.R.; Frank, D.; Rappert, S.; Zeng, A.P.
    Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum (2014), Appl. Environ. Microbiol., 80, 1388-1393.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-RIL cells Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
K653G the mutation dramatically reduces the enzyme activity Corynebacterium glutamicum
K813G the mutation does not significantly alter the enzyme activity Corynebacterium glutamicum
N917G the mutation does not significantly alter the enzyme activity and results in about 37% improved L-lysine production Corynebacterium glutamicum
R620G the mutation dramatically reduces the enzyme activity Corynebacterium glutamicum
R873G the mutation does not significantly alter the enzyme activity Corynebacterium glutamicum
S869G the mutation does not significantly alter the enzyme activity Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
aspartate the enzyme loses about 95% activity when the aspartate concentration is more than 2 mM Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6 6 phosphoenolpyruvate mutant enzyme R873G, at pH 8.5 and 25°C Corynebacterium glutamicum
0.62
-
phosphoenolpyruvate wild type enzyme, at pH 8.5 and 25°C Corynebacterium glutamicum
0.68
-
phosphoenolpyruvate mutant enzyme S869G, at pH 8.5 and 25°C Corynebacterium glutamicum
0.69
-
phosphoenolpyruvate mutant enzyme N917G, at pH 8.5 and 25°C Corynebacterium glutamicum
0.78
-
phosphoenolpyruvate mutant enzyme K813G, at pH 8.5 and 25°C Corynebacterium glutamicum
2.6
-
HCO3- mutant enzyme N917G, at pH 8.5 and 25°C Corynebacterium glutamicum
2.8
-
HCO3- mutant enzyme R873G, at pH 8.5 and 25°C Corynebacterium glutamicum
2.8
-
HCO3- wild type enzyme, at pH 8.5 and 25°C Corynebacterium glutamicum
2.9
-
HCO3- mutant enzyme S869G, at pH 8.5 and 25°C Corynebacterium glutamicum
3.1
-
HCO3- mutant enzyme K813G, at pH 8.5 and 25°C Corynebacterium glutamicum
3.3
-
HCO3- mutant enzyme R620G, at pH 8.5 and 25°C Corynebacterium glutamicum
3.31
-
phosphoenolpyruvate mutant enzyme K653G, at pH 8.5 and 25°C Corynebacterium glutamicum
3.5
-
HCO3- mutant enzyme K653G, at pH 8.5 and 25°C Corynebacterium glutamicum
4.88
-
phosphoenolpyruvate mutant enzyme R620G, at pH 8.5 and 25°C Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + HCO3- Corynebacterium glutamicum
-
phosphate + oxaloacetate
-
?
phosphoenolpyruvate + HCO3- Corynebacterium glutamicum LC298
-
phosphate + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum LC298
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + HCO3-
-
Corynebacterium glutamicum phosphate + oxaloacetate
-
?
phosphoenolpyruvate + HCO3-
-
Corynebacterium glutamicum LC298 phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
PEPC
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Corynebacterium glutamicum