Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-RIL cells | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
K653G | the mutation dramatically reduces the enzyme activity | Corynebacterium glutamicum |
K813G | the mutation does not significantly alter the enzyme activity | Corynebacterium glutamicum |
N917G | the mutation does not significantly alter the enzyme activity and results in about 37% improved L-lysine production | Corynebacterium glutamicum |
R620G | the mutation dramatically reduces the enzyme activity | Corynebacterium glutamicum |
R873G | the mutation does not significantly alter the enzyme activity | Corynebacterium glutamicum |
S869G | the mutation does not significantly alter the enzyme activity | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartate | the enzyme loses about 95% activity when the aspartate concentration is more than 2 mM | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | 6 | phosphoenolpyruvate | mutant enzyme R873G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
0.62 | - |
phosphoenolpyruvate | wild type enzyme, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
0.68 | - |
phosphoenolpyruvate | mutant enzyme S869G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
0.69 | - |
phosphoenolpyruvate | mutant enzyme N917G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
0.78 | - |
phosphoenolpyruvate | mutant enzyme K813G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
2.6 | - |
HCO3- | mutant enzyme N917G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
2.8 | - |
HCO3- | mutant enzyme R873G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
2.8 | - |
HCO3- | wild type enzyme, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
2.9 | - |
HCO3- | mutant enzyme S869G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
3.1 | - |
HCO3- | mutant enzyme K813G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
3.3 | - |
HCO3- | mutant enzyme R620G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
3.31 | - |
phosphoenolpyruvate | mutant enzyme K653G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
3.5 | - |
HCO3- | mutant enzyme K653G, at pH 8.5 and 25°C | Corynebacterium glutamicum | |
4.88 | - |
phosphoenolpyruvate | mutant enzyme R620G, at pH 8.5 and 25°C | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | Corynebacterium glutamicum | - |
phosphate + oxaloacetate | - |
? | |
phosphoenolpyruvate + HCO3- | Corynebacterium glutamicum LC298 | - |
phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum LC298 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Corynebacterium glutamicum | phosphate + oxaloacetate | - |
? | |
phosphoenolpyruvate + HCO3- | - |
Corynebacterium glutamicum LC298 | phosphate + oxaloacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PEPC | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Corynebacterium glutamicum |