Literature summary for 4.1.1.31 extracted from

Dharmarajan, L.; Kraszewski, J.L.; Mukhopadhyay, B.; Dunten, P.W.
Structure of an archaeal-type phosphoenolpyruvate carboxylase sensitive to inhibition by aspartate (2011), Proteins, 79, 1820-1829.

Search for more literature for 4.1.1.31

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	not activated by D-glucose 6-phosphate	Clostridium perfringens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 1.25-1.5 M sodium malonate, pH 7.0, as precipitant	Clostridium perfringens

Inhibitors

Inhibitors	Comment	Organism	Structure
L-Asp	L-Asp competitively inhibits the enzyme with respect to the substrate, Mg2+-phosphoenolpyruvate	Clostridium perfringens
malonate	weak inhibitor	Clostridium perfringens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	phosphoenolpyruvate	in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C	Clostridium perfringens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion	Clostridium perfringens
Mg2+	required for activity, PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion	Clostridium perfringens
Mn2+	PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion	Clostridium perfringens

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	Q8XLE8	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography and gel filtration	Clostridium perfringens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + HCO3-	-	Clostridium perfringens	phosphate + oxaloacetate	-	?

Subunits

Subunits	Comment	Organism
tetramer	X-ray crystallography, analytical ultracentrifugation, or sedimentation velocity analysis	Clostridium perfringens

Synonyms

Synonyms	Comment	Organism
archaeal-type phosphoenolpyruvate carboxylase	-	Clostridium perfringens
PEPC	-	Clostridium perfringens
PepcA	-	Clostridium perfringens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.2	-	L-Asp	in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C	Clostridium perfringens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)