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Literature summary for 4.1.1.31 extracted from

  • Gowik, U.; Engelmann, S.; Blaesing, O.E.; Raghavendra, A.S.; Westhoff, P.
    Evolution of C4 phosphoenolpyruvate carboxylase in the genus Alternanthera: gene families and the enzymatic characteristics of the C4 isozyme and its orthologs in C3 and C3/C4 Alternantheras (2006), Planta, 223, 359-368.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 6-phosphate
-
Alternanthera pungens
D-glucose 6-phosphate
-
Alternanthera ficoidea
D-glucose 6-phosphate
-
Alternanthera sessilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Alternanthera pungens
expressed in Escherichia coli Alternanthera ficoidea
expressed in Escherichia coli Alternanthera sessilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information K0.5 (phosphoenolpyruvate) (without 5 mM glucose 6-phosphate): 0.036 mM, K0.5 (phosphoenolpyruvate) (+ 5 mM glucose 6-phosphate): 0.013 mM Alternanthera sessilis
additional information
-
additional information K0.5 (phosphoenolpyruvate) (without 5 mM glucose 6-phosphate): 0.042 mM, K0.5 (phosphoenolpyruvate) (+ 5 mM glucose 6-phosphate): 0.025 mM Alternanthera ficoidea
additional information
-
additional information K0.5 (phosphoenolpyruvate) (without 5 mM glucose 6-phosphate): 0.157 mM, K0.5 (phosphoenolpyruvate) (+ 5 mM glucose 6-phosphate): 0.02 mM Alternanthera pungens

Organism

Organism UniProt Comment Textmining
Alternanthera ficoidea Q1XAT8
-
-
Alternanthera pungens Q1XAT9
-
-
Alternanthera sessilis Q1XAT7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf strong expression detected by Northern blot-analysis Alternanthera pungens
-
leaf weak expression detected by Northern blot-analysis Alternanthera ficoidea
-
leaf weak expression detected by Northern blot-analysis Alternanthera sessilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Alternanthera pungens phosphate + oxaloacetate
-
?
phosphoenolpyruvate + CO2
-
Alternanthera ficoidea phosphate + oxaloacetate
-
?
phosphoenolpyruvate + CO2
-
Alternanthera sessilis phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
PEPCase
-
Alternanthera pungens
PEPCase
-
Alternanthera ficoidea
PEPCase
-
Alternanthera sessilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
phosphoenolpyruvate without 5 mM glucose 6-phosphate Alternanthera sessilis
16
-
phosphoenolpyruvate + 5 mM glucose 6-phosphate Alternanthera sessilis
33
-
phosphoenolpyruvate + 5 mM D-glucose 6-phosphate Alternanthera ficoidea
33
-
phosphoenolpyruvate without 5 mM D-glucose 6-phosphate Alternanthera ficoidea
38
-
phosphoenolpyruvate + 5 mM D-glucose 6-phosphate Alternanthera pungens
38
-
phosphoenolpyruvate without 5 mM D-glucose 6-phosphate Alternanthera pungens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Alternanthera pungens
8
-
assay at Alternanthera ficoidea
8
-
assay at Alternanthera sessilis