Literature summary for 4.1.1.31 extracted from

Tripodi, K.E.; Turner, W.L.; Gennidakis, S.; Plaxton, W.C.
In vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase isoforms in endosperm of developing castor oil seeds (2005), Plant Physiol., 139, 969-978.

Search for more literature for 4.1.1.31

Activating Compound

Activating Compound	Comment	Organism	Structure
glucose 6-phosphate	activates PEPC1	Ricinus communis

Inhibitors

Inhibitors	Comment	Organism	Structure
aspartate	IC50 of phospho-PEPC1: 0.35 mM, IC50 of dephospho-PEPC1: 0.32 mM; IC50 of phospho-PEPC2: 2.6 mM, IC50 of dephospho-PEPC2: 4.5 mM, enzyme form PEPC2	Ricinus communis
glutamate	IC50 of phospho-PEPC1: 2.1 mM, IC50 of dephospho-PEPC1: 2.2 mM; IC50 of phospho-PEPC2: 4.1 mM, IC50 of dephospho-PEPC2: 7.0 mM, enzyme form PEPC2	Ricinus communis
malate	IC50 of phospho-PEPC1: 0.075 mM, IC50 of dephospho-PEPC1: 0.029 mM; IC50 of phospho-PEPC2: 0.57 mM, IC50 of dephospho-PEPC2: 1.47 mM, enzyme form PEPC2	Ricinus communis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	phosphoenolpyruvate	dephospho-PEPC2	Ricinus communis
0.55	-	phosphoenolpyruvate	phospho-PEPC2	Ricinus communis
0.55	-	phosphoenolpyruvate	phosphoPEPC1	Ricinus communis
0.92	-	phosphoenolpyruvate	dephosphoPEPC1	Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	x * 107000 + x * 64000, enzyme form PEPC2	Ricinus communis
107000	-	4 * 107000	Ricinus communis
107000	-	x * 107000 + x * 64000, enzyme form PEPC2	Ricinus communis

Organism

Organism	UniProt	Comment	Textmining
Ricinus communis	-	L. var. Baker 296	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	Ser6 is phosphorylated. Ser6 phosphorylation of the p107 subunit increases KM-value of PEPC2 for phosphoenolpyruvate and sensitivity to L-malate, glutamic acid, and aspartic acid inhibition. Phosphorylation of subunit p107 is promoted during development of Ricinus communis but disappears during desiccation. The p107 stage VII becomes fully dephosphorylated in plants 48 h following excision of Ricinus communis pods or following 72 h of dark treatment of intact plants	Ricinus communis
phosphoprotein	Ser6 is phosphorylated. Ser6 phosphorylation of the p107 subunit increases PEPC1 activity at pH 7.3 by decreasing its KM for phosphoenolpyruvate and sensitivity to L-malate inhibition, while enhancing glucose 6-phosphate activation	Ricinus communis

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + HCO3-	-	Ricinus communis	phosphate + oxaloacetate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 107000	Ricinus communis
octamer	x * 107000 + x * 64000, enzyme form PEPC2	Ricinus communis

Synonyms

Synonyms	Comment	Organism
PEPC1	-	Ricinus communis
PEPC2	-	Ricinus communis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.029	-	IC50 of dephospho-PEPC1: 0.029 mM	Ricinus communis	malate
0.075	-	IC50 of phospho-PEPC1: 0.075 mM	Ricinus communis	malate
0.32	-	IC50 of dephospho-PEPC1: 0.32 mM	Ricinus communis	aspartate
0.35	-	IC50 of phospho-PEPC1: 0.35 mM	Ricinus communis	aspartate
0.57	-	IC50 of phospho-PEPC2: 0.57 mM	Ricinus communis	malate
1.47	-	, IC50 of dephospho-PEPC2: 1.47 mM, enzyme form PEPC2	Ricinus communis	malate
2.1	-	IC50 of phospho-PEPC1: 2.1 mM	Ricinus communis	glutamate
2.2	-	IC50 of dephospho-PEPC1: 2.2 mM	Ricinus communis	glutamate
2.6	-	IC50 of phospho-PEPC2: 2.6 mM	Ricinus communis	aspartate
4.1	-	IC50 of phospho-PEPC2: 4.1 mM	Ricinus communis	glutamate
4.5	-	IC50 of dephospho-PEPC2: 4.5 mM, enzyme form PEPC2	Ricinus communis	aspartate
7	-	IC50 of dephospho-PEPC2: 7.0 mM, enzyme form PEPC2	Ricinus communis	glutamate

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)