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Literature summary for 4.1.1.31 extracted from
- Purification and characterization of phosphoenolpyruvate carboxylase from the hyperthermophilic archaeon Methanothermus sociabilis (1996), FEBS Lett., 392, 148-152.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| fructose 1,6-bisphosphate | no effect | Methanothermus sociabilis |  |
| glucose 6-phosphate | no effect | Methanothermus sociabilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
phosphoenolpyruvate | - |
Methanothermus sociabilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanothermus sociabilis | |
| Mn2+ | activates | Methanothermus sociabilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Methanothermus sociabilis |
| 240000 | - |
gel filtration | Methanothermus sociabilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermus sociabilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermus sociabilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
- |
Methanothermus sociabilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Methanothermus sociabilis | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Methanothermus sociabilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Methanothermus sociabilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 95 | 60°C: about 30% of maximal activity, 95°C: about 40% of maximal activity | Methanothermus sociabilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
2 h, no loss of activity | Methanothermus sociabilis |
| 80 | - |
50% loss of activity after 240 min | Methanothermus sociabilis |
| 90 | - |
50% loss of activity after 20 min | Methanothermus sociabilis |
| 100 | - |
50% loss of activity after 1 min | Methanothermus sociabilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Methanothermus sociabilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | no effect | Methanothermus sociabilis | |
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